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ABN1712 Anti-NRL (Neural Retina Leucine Zipper)

Anti-NRL (Neural Retina Leucine Zipper), Cat. No. ABN1712, is a highly specific rabbit polyclonal antibody that targets Neural retina-specific leucine zipper protein and has been tested for use in Immunofluorescence and Western Blotting.

Anti-NRL (Neural Retina Leucine Zipper) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: Nrl UniProt Number: P54846
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ABN1712
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MIF, WBRbPurifiedPolyclonal Antibody
      Description
      Catalogue NumberABN1712
      DescriptionAnti-NRL (Neural Retina Leucine Zipper)
      Alternate Names
      • NRL
      Background InformationNeural retina-specific leucine zipper protein (UniProt: P54846; also known as NRL) is encoded by the Nrl gene (Gene ID: 18185) in murine species. NRL is a bZIP transcription factor of the MAF family that regulates the expression of several rod-specific genes, including RHO and phosphodiesterase 6B. NRL is reported to be present in the nuclei of developing and mature rods but not cones. It interacts with the homeodomain protein CRX and synergistically regulates rhodopsin expression. It is also reported to interact with FIZ1 and this interaction can repress transactivation. It consists of a minimal transactivation domain (aa 30-93), a basic motif (aa 159-185), and a leucine-zipper (aa 187-208). The minimal transactivation domain (MTD) is conserved across the MAF family and it may activate transcription by recruiting TBP and associated factors at the promoters of target genes. In developing mouse retina, Nrl expression is reported to precede the appearance of known markers for differentiated rods. The transcriptional activity of NRL is modulated by sumoylation on RHO and NR2E3 promoters. (Ref.: Prabodha, K et al. (2001). J. Biol. Chem 276 (39): 36824-30).
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in PBS with 0.05% sodium azide and 50% glycerol.
      Quality LevelMQ100
      Applications
      ApplicationAnti-NRL (Neural Retina Leucine Zipper), Cat. No. ABN1712, is a highly specific rabbit polyclonal antibody that targets Neural retina-specific leucine zipper protein and has been tested for use in Immunofluorescence and Western Blotting.
      Key Applications
      • Immunofluorescence
      • Western Blotting
      Application NotesImmunofluorescence Analysis: A 1:100-1:1,000 dilution from a representative lot detected NRL (Neural Retina Leucine Zipper) in frozen mouse retina tissue (Courtesy of Dr. Tiansen Li at NIH).

      Western Blotting Analysis: A 1:1,000-1:2,000 dilution from a representative lot detected NRL (Neural Retina Leucine Zipper) in mouse retinal tissue lysate (Courtesy of Dr. Tiansen Li at NIH).
      Biological Information
      ImmunogenHis-tagged recombinant protein fragment corresponding to 108 amino acids from the N-terminal half of murine neural retina-specific leucine zipper protein.
      Epitopeunknown
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody recognizes murine neural retina-specific leucine zipper protein. It iargets an epitope within 108 amino acids from the N-terminal half.
      IsotypeIgG
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Nrl
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~31 kDa observed; 26.08 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse retinal tissue lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected NRL (Neural Retina Leucine Zipper) in mouse retinal tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.

      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABN1712 04054839339578