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MABC1183 Anti-PME1 Antibody, clone 8A6-F8

Detect Protein phosphatase methylesterase 1 using this mouse monoclonal Anti-PME1 Antibody, clone 8A6-F8, Cat. No. MABC1183. It is used in Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

Anti-PME1 Antibody, clone 8A6-F8 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Gene Symbol: Ppme1, Pme1 UniProt Number: Q8BVQ5
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MABC1183
200 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RIP, WB, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABC1183
      DescriptionAnti-PME1 Antibody, clone 8A6-F8
      Alternate Names
      • PME-1
      • Protein phosphatase methylesterase 1
      Background InformationProtein phosphatase methylesterase 1 (UniProt: Q8BVQ5; also known as PME1; EC:3.1.1.89) is encoded by the Ppme1 (also known as Pme1) gene (Gene ID: 72590) in murine species. PME1 is a member of the AB hydrolase family that shows ubiquitous distribution, but is highly expressed in testis and brain. PME-1 is shown to be PMSF-resistant and okadaic acid-sensitive methyltransferase. PME-1 contains a motif found in lipases that utilize a catalytic triad-activated serine as their active site nucleophile, and has other scattered homology with other lipases in which this motif is conserved. PME1 catalyzes the demethylation of proteins that have been reversibly carboxymethylated. PME1 is shown to demethylate and inactivate protein phosphatase 2A (PP2A). Its binding to the catalytic site of PP2A displaces manganese ion that results in the inactivation of PP2A. Targeted disruption of the Ppme1 gene is shown to cause perinatal lethality in mice, resulting from a virtually complete loss of the demethylated form of PP2A in the nervous system and peripheral tissues.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect Protein phosphatase methylesterase 1 using this mouse monoclonal Anti-PME1 Antibody, clone 8A6-F8, Cat. No. MABC1183. It is used in Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected PME1 in human testis and human cerebral cortex tissue.

      Immunoprecipitation Analysis: A representative lot detected PME1 in NIH3T3 mouse fibroblasts, wild-type, but not in PME1 knock-out mouse embryonic fibroblasts. (Courtesy of Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna Austria).

      Western Blotting Analysis: A 1:50 dilution from a representative lot detected PME1 in HeLa, NIH3T3, Rat1, BHK21, and CV-1 lysates. (Courtesy of Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna Austria).
      Biological Information
      ImmunogenHis-tagged full length recombinant mouse Protein phosphatase methylesterase 1.
      Clone8A6-F8
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 8A6-F8 specifically stains PME-1 in paraffin sections of human pancreas, testis, and cerebral cortex.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteHuman, Mouse, Rat.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Ppme1
      • Pme1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight42.25 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in human pancreas tissue.

      Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected PME1 in human pancreas tissue.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABC1183 04054839168673