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ABE69 Anti-PTIP Antibody

Anti-PTIP Antibody is a Chicken Polyclonal Antibody for detection of PTIP also known as PAX transactivation activation domain-interacting protein, protein encoded by CAG trinucleotide repeats & has been validated in WB, IHC & IF.

Anti-PTIP Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Chromatin Biology Gene Symbol: PAXIP1L, PTIP, PAXIP1, TNRC2, CAGF28, PACIP1, CAGF29 UniProt Number: Q6ZW49
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ABE69
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, IHC, IFChAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE69
      DescriptionAnti-PTIP Antibody
      Alternate Names
      • PAX interacting (with transcription-activation domain) protein 1
      • PAX transactivation activation domain-interacting protein
      • protein encoded by CAG trinucleotide repeats
      • PAX transcription activation domain interacting protein 1 like
      Background InformationPAX transactivation activation domain-interacting protein (PTIP) is a ubiquitous nuclear protein composed of 5 tandem BRCA1 carboxyl-terminal (BRCT) domains. PTIP was initially identified as an interacting, transactivation protein that binds to the C-terminal end of Pax2. Since its discovery, studies have shown PTIP to bind several Pax proteins, playing a critical role in response to DNA damage. It also has activity as a phosphoserine/ phosphothreonine-specific binding module, and can discern ATM and ATR gamma irradiation induced phosphorylated substrates. PTIP is involved in early cellular development and is likely to have a role in cell proliferation as well.
      References
      Product Information
      FormatAffinity Purified
      Control
      • A431 cell lysate
      PresentationPurified chicken polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-PTIP Antibody is a Chicken Polyclonal Antibody for detection of PTIP also known as PAX transactivation activation domain-interacting protein, protein encoded by CAG trinucleotide repeats & has been validated in WB, IHC & IF.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • Immunofluorescence
      Application NotesImmunohistochemistry Analysis: A representative lot was used by an independent laboratory in IH (Patel, S., et al. (2007).

      Immunofluorescence Analysis: A represenative lot was used by an independent laboratory in IF (Lechner, M., et al. (2000).
      Biological Information
      ImmunogenHistidine-tagged recombinant protein corresponding to human PTIP.
      HostChicken
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene is a member of the paired box (PAX) gene family and encodes a nuclear protein with six BRCT (breast cancer carboxy-terminal) domains. This protein plays a critical role in maintaining genome stability, condensation of chromatin and progression through mitosis. [provided by RefSeq].
      Gene Symbol
      • PAXIP1L
      • PTIP
      • PAXIP1
      • TNRC2
      • CAGF28
      • PACIP1
      • CAGF29
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Functions as a key component of the DNA damage response. Functions as phosphoserine or phosphothreonine-specific binding modules. Recognizes substrates phosphorylated by the kinases ataxia telangiectasia-mutated (ATM), ataxia telangiectasia and RAD3-related (ATR) in response to gamma irradiation. May play a role in PAX2-dependent transcriptional regulation. Plays a role in early development, most probably in cell proliferation.

      SUBUNIT STRUCTURE: Interacts with the C-terminal transactivation domain of PAX2 (By similarity). Interacts with C16orf53/PA1; the interaction is direct. Component of the MLL3/MLL4 complex, at least composed of MLL3, MLL4, ASH2L, RBBP5, DPY30, WDR5, NCOA6, KDM6A, PAXIP1/PTIP and C16orf53/PA1. Some fraction of the complex contains MLL2 instead of MLL4 and is named MLL2/MLL3 complex.

      SUBCELLULAR LOCATION: Nucleus membrane; Peripheral membrane protein; Matrix side (By similarity).

      DOMAIN: The BRCT domains function as a single module and are necessary and sufficient for phospho-specific binding.

      SEQUENCE SIMILARITIES: Contains 5 BRCT domains.

      SEQUENCE CAUTION: The sequence AAB91434.1 differs from that shown. Reason: Frameshift at positions 609 and 628.

      The sequence AAP21865.1 differs from that shown. Reason: Erroneous gene model prediction.
      Molecular Weight~120 kDa observed.
      Other isoforms may be observed in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in A431 cell lysate.

      Western Blot Analysis: A 1:1,000 dilution of this antibody detected PTIP in 10 µg of A431 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABE69 04053252413117