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MABF2784-100UG Anti-SARS-CoV-2 Spike RBD Antibody, clone 6E4-C5

Anti-SARS-CoV-2 Spike RBD, clone 6E4-C5, Cat. No. MABF2784, is a mouse monoclonal antibody that detects spike glycoprotein of SARS-CoV-2 and is tested for use in ELISA, Immunoprecipitation, Neutralizing, and Western Blotting.

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MABF2784-100UG
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABF2784-100UG
      DescriptionAnti-SARS-CoV-2 Spike RBD Antibody, clone 6E4-C5
      Alternate Names
      • Spike glycoprotein
      • S glycoprotein
      • E2
      • Peplomer protein
      Background InformationSpike glycoprotein (UniProt: P0DTC2; also known as S glycoprotein, E2, Peplomer protein) is encoded by the S (also known as 2) gene (Gene ID: 43740568) in Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The SARS-CoV-2 is a positive-strand RNA virus that causes severe respiratory syndrome in human. The mature SARS-CoV-2 contains 4 structural proteins: Envelope (E), Membrane (M), Nucleocapsid (N), and the Spike protein (S). E and M proteins help in viral assembly and N protein is needed for RNA synthesis. The S protein is a single-pass type I, homotrimeric, membrane glycoprotein that is responsible for virus binding and entry into host cell. It is synthesized with a signal peptide (aa 1-12), which is subsequently cleaved off to generate the mature protein that contains an extracellular domain (aa 13-1213), a transmembrane domain (aa 1214-1234), and a cytoplasmic domain 1235-1273). The S protein is further processed into S1 (aa 13-685) and S2 (aa 686-1273) subunits by host cell furin. The presence of a furin polybasic cleavage site in S protein from SARS-CoV-2 sets it apart from S protein in SARS-CoV that possesses a monobasic S1/S2 cleavage site. The S1 subunit has the receptor binding domain (RBD; aa 319-541) that mediates entry of SARS-CoV-2 into sensitive cells through the peptidase domain of host Angiotensin-converting enzyme 2 (ACE2) with high affinity (KD = 15 nM). The S2 domain (aa 686-1213), which is reported to be well conserved, is responsible for membrane fusion. (Ref.: Abe, KT., et al. (2020). JCI Insight. 5(19); e142362).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in PBS without azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-SARS-CoV-2 Spike RBD, clone 6E4-C5, Cat. No. MABF2784, is a mouse monoclonal antibody that detects spike glycoprotein of SARS-CoV-2 and is tested for use in ELISA, Immunoprecipitation, Neutralizing, and Western Blotting.
      Key Applications
      • Western Blotting
      • Neutralizing
      • Immunoprecipitation
      • ELISA
      Application NotesTested Applications

      Neutralizing: A representative lot of this antibody reduced binding of SARS-CoV-2 RBD to ACE2 receptor in a Binding Interference Assay. (Courtesy: Kento Abe and Anne-Claude Gingras, University of Toronto, Canada; Abe, KT., et al. (2020). JCI Insight. 5(19); e142362).

      ELISA Analysis: Various dilutions of a hybridoma culture supernatant for clone 6E4-C5 detected native SARS-CoV-2 spike protein. ( (Courtesy of Stefan Schüchner, Ingrid Mudrak, Ingrid Frohner, and E. Ogris (Max Perutz Labs, Medical University of Vienna, Austria).

      Immunoprecipitation Analysis: A representative lot immunoprecipitated spike glycoprotein of SARS-CoV-2. (Courtesy of Stefan Schüchner, Ingrid Mudrak, Ingrid Frohner, and E. Ogris (Max Perutz Labs, Medical University of Vienna, Austria).

      Western Blotting Analysis: A 1:400 dilution from a representative lot detected spike glycoprotein in whole cell lysate of HEK293T cells expressing full-length spike protein of SARS-CoV-2, but not in untransfected cells. (Courtesy of Stefan Schüchner, Ingrid Mudrak, Ingrid Frohner, and E. Ogris (Max Perutz Labs, Medical University of Vienna, Austria).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenHis-tagged recombinant fragment corresponding to 223 amino acids from receptor binding domain of SARS-CoV-2.
      EpitopeExtracellular domain
      Clone6E4-C5
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 6E4-C5 is a mouse monoclonal antibody that detects the spike glycoprotein of SARS-CoV-2. It targets an epitope within the receptor binding domain.
      IsotypeIgG1κ
      Species Reactivity
      • Virus
      Species Reactivity NoteSARS-CoV-2
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • S
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~200 kDa observed; 141.18 kDa calculated.. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in lysate from HEK293T cells expressing full-length spike protein of SARS-CoV-2.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected spike glycoprotein in lysate from HEK293T cells expressing full-length SARS-CoV-2 spike protein, but not in lysate from wild type HEK393T cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF2784-100UG 04065267403908