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AB5208-50UL Anti-Sodium Channel Antibody, Voltage Gated, Brain Type III, pain

Detect Sodium Channel using this Anti-Sodium Channel Antibody, Voltage Gated, Brain Type III, pain validated for use in IH & WB.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Ion Channels & Transporters Gene Symbol: SCN3A, Nav1.3, KIAA1356, NAC3 UniProt Number: Q9NY46
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AB5208-50UL
50 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, RIHC, WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB5208-50UL
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Sodium Channel Antibody, Voltage Gated, Brain Type III, pain
      Alternate Names
      • Nav1.3
      • SCN3A
      References
      Product Information
      FormatAffinity Purified
      Control
      • CONTROL ANTIGEN: Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 0.75 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
      PresentationAffinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA as a stabilizer and 0.05% sodium azide as a preservative. Reconstitute with 50 mL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
      Quality LevelMQ100
      Applications
      ApplicationDetect Sodium Channel using this Anti-Sodium Channel Antibody, Voltage Gated, Brain Type III, pain validated for use in IH & WB.
      Key Applications
      • Immunohistochemistry
      • Western Blotting
      Application NotesAll procedures that are going to receive a full-length protein should be performed at 4°C, and the following protease inhibitor mixture should be used: pepstatin A (1 μg/mL), leupeptin (1 μg/mL), aprotinin (1 μg/mL), Pefabloc SC (0.2 mM), benzamidine (0.1 mg/mL), and calpain inhibitors I and II (8 μg/mL each).

      Western blot: 1:200 using ECL on newborn rat brain membranes.

      Dilutions should be made using a carrier protein such as BSA (1-3%).

      Immunohistochemistry: on fixed frozen sections.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenPurified peptide of alpha subunit (amino acids 511-524) of rat Nav1.3

      ( type III voltage-gated sodium channel VGSC)
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes Nav1.3 (type III a subunit of VGSC, Scn3a). Does not cross react with any other sodium channel antigens tested so far.
      Species Reactivity
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryVoltage-gated sodium channels are transmembrane glycoprotein complexes composed of a large alpha subunit with 24 transmembrane domains and one or more regulatory beta subunits. They are responsible for the generation and propagation of action potentials in neurons and muscle. This gene encodes one member of the sodium channel alpha subunit gene family, and is found in a cluster of five alpha subunit genes on chromosome 2. Multiple transcript variants encoding different isoforms have been found for this gene.
      Gene Symbol
      • SCN3A
      • Nav1.3
      • KIAA1356
      • NAC3
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q9NY46 # Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient.
      SIZE: 2000 amino acids; 226294 Da
      SUBUNIT: The sodium channel consists of a large polypeptide and 2- 3 smaller ones. This sequence represents a large polypeptide. Interacts with NEDD4L.
      SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
      DOMAIN: SwissProt: Q9NY46 The sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1,S2,S3,S5,S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position.
      PTM: May be ubiquitinated by NEDD4L; which would promote its endocytosis.
      SIMILARITY: Belongs to the sodium channel family. & Contains 1 IQ domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size50 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AB5208-50UL 04053252330995

      Documentation

      Anti-Sodium Channel Antibody, Voltage Gated, Brain Type III, pain SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Sodium Channel Antibody, Voltage Gated, Brain Type III, pain Certificates of Analysis

      TitleLot Number
      RABBIT ANTI- Nav1.3 AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2127746 2127746
      RABBIT ANTI- Nav1.3 AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2143445 2143445
      RABBIT ANTI- Nav1.3 AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2383449 2383449
      RABBIT ANTI- Nav1.3 AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2426493 2426493
      RABBIT ANTI- Nav1.3 - 3185054 3185054
      RABBIT ANTI- Nav1.3 - 3697403 3697403
      RABBIT ANTI- Nav1.3 - 4252120 4252120
      RABBIT ANTI- Nav1.3 -2802525 2802525
      RABBIT ANTI- Nav1.3 AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2049189 2049189
      RABBIT ANTI- Nav1.3 AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2283358 2283358

      References

      Reference overviewPub Med ID
      Reduced sodium current in GABAergic interneurons in a mouse model of severe myoclonic epilepsy in infancy
      Yu, Frank H, et al.
      Nat. Neurosci., 9:1142-1149 (2006)  2006

      		16921370 16921370
      Distinct subcellular localization of different sodium channel alpha and beta subunits in single ventricular myocytes from mouse heart
      Maier, Sebastian K G, et al
      Circulation, 109:1421-7 (2004)  2004

      		15007009 15007009

      Data Sheet

      Title
      RABBIT ANTI- Nav1.3 AFFINITY PURIFIED POLYCLONAL ANTIBODY