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ABD29 Anti-Twist1 (Twist-related protein 1) Antibody

Detect Twist1 (Twist-related protein 1) using this Anti-Twist1 (Twist-related protein 1) Antibody validated for use in IH(P) & IC.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Stem Cell Research Research Sub-Category: Transcription Factors Gene Symbol: TWIST1, BHLHA38, TWIST UniProt Number: Q15672
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ABD29
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, Ca, Po, Rhesus Macaque, Chp, EqIH(P), ICCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABD29
      DescriptionAnti-Twist1 (Twist-related protein 1) Antibody
      Alternate Names
      • Twist-related protein 1
      • Class A basic helix-loop-helix protein 38
      • bHLHa38
      • H-twist
      Background InformationTwist1 is the basic helix-loop-helix transcription factor that regulates embryonic progenitor and transformed tumor cell proliferation, migration and differentiation and is also important in limb development. It has been shown that twist1 expression is high in patients suffering from rheumatic inflammatory disorders with chronically activated T helper lymphocytes and may be used as a biomarker for chronic t-cell activation. Mutations in twist1 are often associated with craniofacial and limb abnormalities in humans with Saethre-Chotzen syndrome. Twist1 dimer affinity can modify the DNA binding affinity. Twist1 has also been implicated as a promoter of tumor growth, invasion and anti-cancer-drug resistance.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Ductal breast fibroadenoma and stratified squamous epithelium and cartilage of human trachea tissues
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect Twist1 (Twist-related protein 1) using this Anti-Twist1 (Twist-related protein 1) Antibody validated for use in IH(P) & IC.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: 1:500 dilution from a representative lot detected Twist1 in NIH/3T3, A431, and HeLa cells.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human Twist1 near the N-terminus.
      EpitopeNear N-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes Twist1 near the N-terminus.
      Species Reactivity
      • Human
      • Mouse
      • Canine
      • Pig
      • Rhesus Macaque
      • Chimpanzee
      • Horse
      Species Reactivity NoteDemonstrated to react with Human and Mouse. Predicted to react with Pig, Rhesus Monkey, Chimpanzee, Equine, Gorilla, and Canine based on 100% sequence homology. Other homologies: Rat (92% sequence homology).
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryBasic helix-loop-helix (bHLH) transcription factors have been implicated in cell lineage determination and differentiation. The protein encoded by this gene is a bHLH transcription factor and shares similarity with another bHLH transcription factor, Dermo1. The strongest expression of this mRNA is in placental tissue; in adults, mesodermally derived tissues express this mRNA preferentially. Mutations in this gene have been found in patients with Saethre-Chotzen syndrome. [provided by RefSeq].
      Gene Symbol
      • TWIST1
      • BHLHA38
      • TWIST
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Acts as a transcriptional regulator. Inhibits myogenesis by sequestrating E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. This interaction probably involves the basic domains of both proteins. Also represses expression of proinflammatory cytokines such as TNFA and IL1B. Regulates cranial suture patterning and fusion. Activates transcription as a heterodimer with E proteins. Regulates gene expression differentially, depending on dimer composition. Homodimers induce expression of FGFR2 and POSTN while heterodimers repress FGFR2 and POSTN expression and induce THBS1 expression. Heterodimerization is also required for osteoblast differentiation.

      SUBUNIT STRUCTURE: Efficient DNA binding requires dimerization with another bHLH protein. Homodimer or heterodimer with E proteins such as TCF3. ID1 binds preferentially to TCF3 but does not interact efficiently with TWIST1 so ID1 levels control the amount of TCF3 available to dimerize with TWIST1 and thus determine the type of dimer formed (By similarity).

      SUBCELLULAR LOCATION: Nucleus.

      TISSUE SPECIFICTY: Subset of mesodermal cells.

      INVOLVEMENT IN DISEASE: Defects in TWIST1 are a cause of Saethre-Chotzen syndrome (SCS) [MIM:101400]; also known as acrocephalosyndactyly type 3 (ACS3). SCS is a craniosynostosis syndrome characterized by coronal synostosis, brachycephaly, low frontal hairline, facial asymmetry, hypertelorism, broad halluces, and clinodactyly.

      Defects in TWIST1 are the cause of Robinow-Sorauf syndrome (RSS) [MIM:180750]; also known as craniosynostosis-bifid hallux syndrome. RSS is an autosomal dominant defect characterized by minor skull and limb anomalies which is very similar to Saethre-Chotzen syndrome.

      Defects in TWIST1 are the cause of craniosynostosis type 1 (CRS1) [MIM:123100]. Craniosynostosis consists of premature fusion of one or more cranial sutures, resulting in an abnormal head shape.

      SEQUENCE SIMILARITIES: Contains 1 basic helix-loop-helix (bHLH) domain.
      Molecular Weight21 kDa calculated
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in ductal breast fibroadenoma and stratified squamous epithelium and cartilage of human trachea tissues.

      Immunohistochemistry Analysis: 1:500 dilution of this antibody detected Twist1 in ductal breast fibroadenoma and stratified squamous epithelium and cartilage of human trachea tissues.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABD29 04053252630279

      Documentation

      Anti-Twist1 (Twist-related protein 1) Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Twist1 (Twist-related protein 1) Antibody Certificates of Analysis

      TitleLot Number
      Anti-Twist1 (Twist-related protein 1) (rabbit polyclonal) Polyclonal Antibody Q2922262
      Anti-Twist1 (Twist-related protein 1) - 2146027 2146027
      Anti-Twist1 (Twist-related protein 1) - 2384748 2384748
      Anti-Twist1 (Twist-related protein 1) - 2420518 2420518
      Anti-Twist1 (Twist-related protein 1) - 2450775 2450775
      Anti-Twist1 (Twist-related protein 1) - 2000059 2000059
      Anti-Twist1 (Twist-related protein 1) - 2039819 2039819
      Anti-Twist1 (Twist-related protein 1) - 2110527 2110527
      Anti-Twist1 (Twist-related protein 1) - 2227181 2227181
      Anti-Twist1 (Twist-related protein 1) - 2301421 2301421
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