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ABE1336-100UG Anti-Wee1

Anti-Wee1, Cat. No. ABE1336-100UG, is a highly specific rabbit polyclonal antibody that targets Wee1-like protein kinase and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-Wee1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: WEE1 UniProt Number: P30291
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ABE1336-100UG
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE1336-100UG
      DescriptionAnti-Wee1
      Alternate Names
      • Wee1-like protein kinase
      • EC: 2.7.10.2
      • WEE1hu
      • Wee1A kinase
      Background InformationWee1-like protein kinase (UniProt: P30291; also known as EC: 2.7.10.2, WEE1hu, Wee1A kinase) is encoded by the WEE1 gene (Gene ID: 7465) in human. Wee1 is a magnesium-dependent nuclear kinase that belongs to the Serine/Threonine family of protein kinases. It serves as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed Cdk1 prior to the onset of mitosis. Two isoforms of Wee1 have been described that are produced by alternative splicing. In isoform 1 the nucleotide binding site is localized to amino acids 305-313 and its protein kinase domain lies within amino acids 299-569. Wee1 specifically phosphorylates and inactivates cyclin B1-complexed Cdk1, reaching a maximum during G2 phase and a minimum as cells enter into M phase. Phosphorylation of cyclin B1-Cdk1 is shown to occur exclusively on Tyrosine 15. Wee1 levels are believed to increase during S and G2 phases due to increase in transcription and a decline in M phase has been attributed to reduced synthesis and higher degradation. Phosphorylation at serine 642 by BRSK1 and BRSK2 in post-mitotic neurons is reported to down-regulate Wee1 activity in polarized neurons. Also, phosphorylation at serine 53 by PLK1 and at serine 123 by Cdk1 generates a signal for its ubiquitination and proteasomal degradation at the onset of G2/M phase.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Wee1, Cat. No. ABE1336-100UG, is a highly specific rabbit polyclonal antibody that targets Wee1-like protein kinase and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesWestern Blotting Analysis: 2 µg/mL from a representative lot detected Wee1 in 10 µg of K562 cell lysate.

      Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Wee1 in human placenta and human testis cancer tissues.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 15 amino acids from the C-terminal region of human Wee1-like protein kinase. The immunogen sequence is conserved between isoforms 1 and 2.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects Wee1-like kinase in human cells. It targets an epitope within 15 amino acids from the C-terminal region.
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Bovine, Mouse, and Rat based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • WEE1
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~72 kDa observed; 71.60 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HepG2 cell lysate.

      Western Blotting Analysis: 2 µg/mL of this antibody detected Wee1 in 10 µg of HepG2 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABE1336-100UG 04054839431678