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ABT58 Anti-phospho PKA-RII (Ser96) Antibody

This Anti-phospho PKA-RII (Ser96) Antibody is validated for use in WB for the detection of phospho PKA-RII (Ser96).

Anti-phospho PKA-RII (Ser96) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure, Signaling Research Sub-Category: Cytoskeletal Signaling, Kinases & Phosphatases Gene Symbol: PRKAR2A, PKR2, PRKAR2 UniProt Number: P12367
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ABT58
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, MWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABT58
      Replaces06-704
      DescriptionAnti-phospho PKA-RII (Ser96) Antibody
      Alternate Names
      • PRKAR2A
      • protein kinase
      • cAMP-dependent, regulatory, type II, alpha
      Background InformationcAMP-dependent protein kinase type II-alpha regulatory subunit (PKA-RIIα) is an enzymatic protein belonging to the cAMP-dependent kinase regulatory chain family, a group of regulatory proteins involved in the PKA signaling pathway. This pathway has been shown to have regulatory roles in development, growth, and mortality of various organisms. PKA-RIIα is a subunit of the PKA type II regulatory chain, one of two PKA isoforms, and is involved in the regulation of membrane interactions through its binding to anchoring proteins, such as MAP2 kinase. Before its activation with cAMP, the enzyme is comprised of two catalytic chains and two regulatory chains, which split into two catalytic monomers and one regulatory dimmer after interacting with cAMP.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Forskolin untreated and treated PC12 cell lysates
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine, pH 7.4, 150 mM NaCl, with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-phospho PKA-RII (Ser96) Antibody is validated for use in WB for the detection of phospho PKA-RII (Ser96).
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to mouse PKA-RIIalpha phosphorylated at Ser96.
      EpitopePhosphorylated Ser96 of PKA-RIIalpha
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThe antibody recognizes PKA-RIIalpha when phosphorylated at Ser96.
      Species Reactivity
      • Human
      • Rat
      • Mouse
      Species Reactivity NoteDemonstrated to react with human and rat. Predicted to react with mouse based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PRKAR2A
      • PKR2
      • PRKAR2
      Modifications
      • Phosphorylation
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.

      SUBUNIT STRUCTURE: The inactive form of the enzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP produces two active catalytic monomers and a regulatory dimer that binds four cAMP molecules. Interacts with AKAP4. Interacts with CBFA2T3 (by similarity).

      TISSUE SPECIFICTY: Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.

      PTM: Phosphorylated by the activated catalytic chain.

      SEQUENCE SIMILARITIES: Belongs to the cAMP-dependent kinase regulatory chain family.

      Contains 2 cyclic nucleotide-binding domains.
      Molecular Weight~ 51 kDa MW observed. The specificity of the target was shown by Lambda phosphatase treatment.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in Forskolin untreated and treated PC12 cell lysates.

      Western Blot Analysis: 1 µg/mL of the antibody detected PKA-RIIalpha (Ser96) in 10 µg of Forskolin untreated and treated PC12 cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABT58 04053252746673

      Documentation

      Anti-phospho PKA-RII (Ser96) Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-phospho PKA-RII (Ser96) Antibody Certificates of Analysis

      TitleLot Number
      Anti-phospho PKA-RII (Ser96) - 2387854 2387854
      Anti-phospho PKA-RII (Ser96) - 2445071 2445071
      Anti-phospho PKA-RII (Ser96) - 2325129 2325129
      Anti-phospho PKA-RII (Ser96) - 3167641 3167641
      Anti-phospho PKA-RII (Ser96) - 4243350 4243350
      Anti-phospho PKA-RII (Ser96) - NRG1774097 NRG1774097
      Anti-phospho PKA-RII (Ser96) -2575529 2575529
      Anti-phospho PKA-RII (Ser96) -2606901 2606901
      Anti-phospho PKA-RII (Ser96) -2657462 2657462
      Anti-phospho PKA-RII (Ser96) -2730801 2730801
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