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AG657 DARPP-32, recombinant, His-tagged

DARPP-32, recombinant, His-tagged MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Western Blotting UniProt Number: Q9UD71 Entrez Gene Number: NM_032192.2, NM_181505.2
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AG657
50 µg  
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      Key Spec Table

      Key ApplicationsEntrez Gene NumberUni Prot Number
      WBNM_032192.2 NM_181505.2 Q9UD71
      Description
      Catalogue NumberAG657
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionDARPP-32, recombinant, His-tagged
      References
      Product Information
      Presentation0.5 mg/mL in Tris-buffered saline containing 40% glycerol.
      Quality LevelMQ100
      Applications
      Key Applications
      • Western Blotting
      Application NotesPhosphatase assays
      Biological Information
      Purity>90% by SDS-PAGE
      SourceRecombinant human DARPP-32, expressed in bacteria with a His-Tag linker.
      Entrez Gene Number
      Entrez Gene SummaryMidbrain dopaminergic neurons play a critical role in multiple brain functions, and abnormal signaling through dopaminergic pathways has been implicated in several major neurologic and psychiatric disorders. One well-studied target for the actions of dopamine is DARPP32. In the densely dopamine- and glutamate-innervated rat caudate-putamen, DARPP32 is expressed in medium-sized spiny neurons (Ouimet and Greengard, 1990 [PubMed 2191086]) that also express dopamine D1 receptors (Walaas and Greengard, 1984 [PubMed 6319627]). The function of DARPP32 seems to be regulated by receptor stimulation. Both dopaminergic and glutamatergic (NMDA) receptor stimulation regulate the extent of DARPP32 phosphorylation, but in opposite directions (Halpain et al., 1990 [PubMed 2153935]). Dopamine D1 receptor stimulation enhances cAMP formation, resulting in the phosphorylation of DARPP32 (Walaas and Greengard, 1984 [PubMed 6319627]); phosphorylated DARPP32 is a potent protein phosphatase-1 (see MIM 176875) inhibitor (Hemmings et al., 1984 [PubMed 6087160]). NMDA receptor stimulation elevates intracellular calcium, which leads to activation of calcineurin and dephosphorylation of phospho-DARPP32, thereby reducing the phosphatase-1 inhibitory activity of DARPP32 (Halpain et al., 1990 [PubMed 2153935]).[supplied by OMIM]
      Gene Symbol
      • PPP1R1B
      • DARPP-32
      • FLJ20940
      • DARPP32
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q9UD71 # Inhibitor of protein-phosphatase 1.
      SIZE: 204 amino acids; 22963 Da
      SUBCELLULAR LOCATION: Cytoplasm.
      PTM: Dopamine- and cyclic AMP-regulated neuronal phosphoprotein. & Phosphorylation of Thr-34 is required for activity (By similarity).
      SIMILARITY: SwissProt: Q9UD71 ## Belongs to the protein phosphatase inhibitor 1 family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -20°C for up to 1 year.
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AG657 04053252734465