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LIF2050 Leukemia Inhibitory Factor from mouse

50 µg, mouse recombinant LIF protein, expressed in E. coli, suitable for stem cell culture

Leukemia Inhibitory Factor from mouse MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Stem Cell Culture, Cell Culture UniProt Number: P09056 Entrez Gene Number: NM_008501
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LIF2050
50 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Key ApplicationsSpeciesSourcePurity
      Cult, CULTMouse mLIF is expressed in E. coli as a fusion protein with GST using the pGEX expression system, cleaved from GST moeity with thrombin and purified by multiple FPLC steps.Greater than 95% by SDS-PAGE. Endotoxin level is less than 0.1 ng per mg of LIF. Tested negative in both aseptic and mycoplasmic tests.
      Description
      Catalogue NumberLIF2050
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionLeukemia Inhibitory Factor from mouse
      OverviewLeukemia Inhibitory Factor (LIF) is a lymphoid factor which promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. LIF has a number of other activities including cholinergic neuron differentiation, control of stem cell pluripotency, bone and fat metabolism, mitogenesis of certain factor dependent cell lines and promotion of megakaryocyte production in vivo. Mouse LIF is a 22 kDa protein containing 203 amino acid residues.
      Alternate Names
      • LIF
      • Differentiation-stimulating Factor
      • D Factor
      References
      Product Information
      PresentationLiquid in PBS, pH 7.4 and 0.02% Tween 20. No preservatives added.
      Quality LevelMQ100
      Applications
      Application50 µg, mouse recombinant LIF protein, expressed in E. coli, suitable for stem cell culture
      Key Applications
      • Stem Cell Culture
      • Cell Culture
      Application NotesQC PROTOCOL

      M1 Bioassay

      1. The M1 bioassay is performed using in vitro semi-solid agar cultures, which contain approximately 300 cells in 1 mL volumes of DME containing 20 % FCS in 0.3% agar.

      2. Add 100 μL of sample or mLIF (10(E4) units/mL in 5% FCS in isotonic saline) in two-fold serial dilutions in duplicate to 35 mm petri dishes.

      3. Add 100 μL of 5% FCS in isotonic saline to two control slides.

      4. Incubate at 37°C in fully humidified atmosphere of 10% CO2 in air for 7 days.

      5. Score the number of colonies that show differentiation (note: 50 units is defined as the amount of activity which results in 50% of the colonies being differentiated).
      Biological Information
      Concentration100 μg/mL
      PurityGreater than 95% by SDS-PAGE. Endotoxin level is less than 0.1 ng per mg of LIF. Tested negative in both aseptic and mycoplasmic tests.
      SourcemLIF is expressed in E. coli as a fusion protein with GST using the pGEX expression system, cleaved from GST moeity with thrombin and purified by multiple FPLC steps.
      Specific ActivityThe activity of mouse LIF is determined by the ability to induce differentiation of murine M1 myeloid leukemic cells. The minimum detectable concentration of mouse LIF in this assay is 0.5 ng/mL. The specific activity is >=1 x 10(E8) units/mg, where 50 units is defined as the amount of mouse LIF required to induce differentiation in 50% of the M1 colonies in 1 mL agar cultures.
      Entrez Gene Number
      Entrez Gene SummaryLeukaemia inhibitory factor is a cytokine that induces macrophage differentiation. Neurotransmitters and neuropeptides, well known for their role in the communication between neurons, are also capable of activating monocytes and macrophages and inducing chemotaxis in immune cells. LIF signals through different receptors and transcription factors. LIF in conjunction with BMP2 acts in synergy on primary fetal neural progenitor cells to induce astrocytes.
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P09056.1 # LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.
      SIZE: 203 amino acids; 22,287 Da
      SUBCELLULAR LOCATION: Secreted.
      SIMILARITY: SwissProt: P09056.1 ## Belongs to the LIF/OSM family.
      Stem Cell Type
      • Mouse Embryonic Stem Cells
      • Neural Stem Cells
      • Hematopoietic Stem Cells
      • Mesenchymal Stem Cells
      • Induced Pluripotent Stem Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      • LABEL LICENSE - USE RESTRICTED TO RESEARCH USE ONLY

        Leukemia Inhibitory Factor ("LIF") is protected by US Patents No. 5,443,825 and 5,750,654 and Canada Patent No. 1,341,581 and 1,341,469 owned by AMRAD Corporation Ltd. and licensed exclusively to Millipore for research uses. The use of this product is limited for research uses only and not for any commercial purposes, such as making or developing other research products from this product or use of this product as a component in a research kit. This product is not to be used in humans or for any diagnostic purposes.

        The purchaser may refuse to accept the conditions of this label license by returning this product unopened and will receive a full refund of the purchase price paid.

        For-profit entities or commercial research entities purchasing this product shall be required to execute a separate commercial license with Millipore within three months of purchase. Researchers may not transfer this product or its derivatives to researchers performing commercial research at other Nonprofit Organizations, or to For-Profit Organizations, without the express written consent of Millipore, and without those entities having an express license from Millipore for the use of the product. Other than as specifically provided herein, a transferee of this product shall have no right to transfer this product, or its derivatives to any other person or entity.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2-8°C for 6-12 months. Further dilutions should be made into buffer or medium to which protein (e.g., 1% BSA) or Tween 20 has been added.
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      LIF2050 04053252410772

      Documentation

      Leukemia Inhibitory Factor from mouse SDS

      Title

      Safety Data Sheet (SDS) 

      Leukemia Inhibitory Factor from mouse Certificates of Analysis

      TitleLot Number
      Recombinant Mouse Leukemia Inhibitory Factor - 2246824 2246824
      Recombinant Mouse Leukemia Inhibitory Factor - 2318885 2318885
      Recombinant Mouse -2618838 2618838
      Recombinant Mouse -2667311 2667311
      Recombinant Mouse -2694548 2694548
      Recombinant Mouse -2712529 2712529
      Recombinant Mouse Leukemia Inhibitory Factor - 3192438 3192438
      Recombinant Mouse Leukemia Inhibitory Factor - 3724736 3724736
      Recombinant Mouse Leukemia Inhibitory Factor - 3824806 3824806
      Recombinant Mouse Leukemia Inhibitory Factor - 3874325 3874325
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      Data Sheet

      Title
      Reprogramming Cell Fate and Function Novel Strategies for iPSC Generation, Characterization, and Differentiation