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FCMAB133F Milli-Mark™ Anti-TRA-2-49 Antibody, clone TRA-2-49/6E, FITC Conjugate

Detect TRA-2-49 using this Milli-Mark Anti-TRA-2-49 Antibody, clone TRA-2-49/6E, FITC Conjugate validated for use in FC.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Stem Cell Research Research Sub-Category: Pluripotent & Early Differentiation Gene Symbol: TRA-2-49
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FCMAB133F
100 tests  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFCMFITCMonoclonal Antibody
      Description
      Catalogue NumberFCMAB133F
      Trade Name
      • Milli-Mark
      DescriptionMilli-Mark™ Anti-TRA-2-49 Antibody, clone TRA-2-49/6E, FITC Conjugate
      Background InformationIn humans, four isoforms of alkaline phosphatase exist: intestinal, placental, placenta-like (germ cell type) and tissue-nonspecific. The tissue-nonspecific isoform of alkaline phosphatase is present in bone, liver and kidney; it differs from the other forms by posttranslational modification.
      Undifferentiated human Embryonal Carcinoma (EC) and Embryonic Stem (ES) cells have been shown to express very high levels of Alkaline Phosphatase isozyme that is indistinguishable from the isozyme found in liver, bone and kidney. Expression levels of AP decrease following stem cell differentiation. This clone can be used to monitor the expression of the Human Liver / Bone / Kidney isozyme of Alkaline Phosphatase (AP), and hence the differentiation status of human EC and ES cells by flow cytometry.
      References
      Product Information
      FormatFITC
      Control
      • 2102 Ep cells.
      PresentationPurified mouse monoclonal IgG1 conjugated to FITC in PBS with 0.1% sodium azide and 15 mg/mL BSA.
      Quality LevelMQ100
      Applications
      ApplicationDetect TRA-2-49 using this Milli-Mark Anti-TRA-2-49 Antibody, clone TRA-2-49/6E, FITC Conjugate validated for use in FC.
      Key Applications
      • Flow Cytometry
      Working Solution Cells / test Total Reaction Volume
      5 μl 5 x 105 Cells / 95 μl PBS 100 μl
      10 μl 1 x 106 Cells / 90 μl PBS 100 μl
      Biological Information
      Immunogen2102Ep human Embryonal Carcinoma Cells
      EpitopeUnknown
      CloneTRA-2-49/6E
      HostMouse
      SpecificityAntibody recognizes TRA-2-49.
      IsotypeIgG1
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • TRA-2-49
      Purification MethodProtein A Purfied
      Molecular Weight 212/80/55 kDa Observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by flow cytometry using 2102 Ep cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8°C protected from light in undiluted aliquots for up to 6 months from date of receipt.
      Packaging Information
      Material Size100 tests
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      FCMAB133F 04053252509797

      Documentation

      Milli-Mark™ Anti-TRA-2-49 Antibody, clone TRA-2-49/6E, FITC Conjugate SDS

      Title

      Safety Data Sheet (SDS) 

      Milli-Mark™ Anti-TRA-2-49 Antibody, clone TRA-2-49/6E, FITC Conjugate Certificates of Analysis

      TitleLot Number
      Milli-Mark™ Anti-TRA-2-49-FITC, -2682521 2682521
      Milli-Mark™ Anti-TRA-2-49-FITC, clone TRA-2-49/6E - 3209743 3209743
      Milli-Mark™ Anti-TRA-2-49-FITC, clone TRA-2-49/6E - 3524808 3524808
      Milli-Mark™ Anti-TRA-2-49-FITC, clone TRA-2-49/6E - 3735419 3735419
      Milli-Mark™ Anti-TRA-2-49-FITC, clone TRA-2-49/6E - 3860256 3860256
      Milli-Mark™ Anti-TRA-2-49-FITC, clone TRA-2-49/6E - 3942774 3942774
      Milli-Mark™ Anti-TRA-2-49-FITC, clone TRA-2-49/6E - 4020601 4020601
      Milli-Mark™ Anti-TRA-2-49-FITC, clone TRA-2-49/6E - 4253435 4253435
      Milli-Mark™ Anti-TRA-2-49-FITC, clone TRA-2-49/6E - 4309898 4309898
      Milli-Mark™ Anti-TRA-2-49-FITC, clone TRA-2-49/6E -2632985 2632985
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