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HTS029RTA Ready-to-Assay™ Human Recombinant H3 Histamine Receptor Frozen Cells

Human H3 GPCR frozen cells for Calcium Flux FLIPR Assays.

Host Cell: Chem-3 Entrez Gene Number: NM_002107.3
HTS029RTA
1 kit  
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      Overview

      Replacement Information

      Key Spec Table

      SpeciesHost CellsProtein Target
      Human Chem-3H3
      Description
      Catalogue NumberHTS029RTA
      Trade Name
      • Ready-to-Assay
      DescriptionReady-to-Assay™ Human Recombinant H3 Histamine Receptor Frozen Cells
      Background InformationMillipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells, resuspends them in media, and allows them to recover for 24 hours. The following day the cells are counted, plated, and assayed for calcium response.

      The histamine H3 receptor was defined pharmacologically to be a presynaptic histamine receptor that binds selectively to R-(α)-methylhistamine and inhibits neurotransmitter release in the central and peripheral nervous systems (Hill et al., 1997). Molecular cloning of the H3 receptor confirmed it to be a Gi-coupled receptor widely expressed at high levels in the brain (Lovenberg et al., 1999). H3 presents a potential therapeutic target for disorders of the central nervous system involving attention, cognition and feeding (Leurs et al., 2005). Millipore’s cloned human H3-expressing cell line is made in the Chem-3 host, which supports high levels of recombinant H3 expression on the cell surface for functional detection via the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at H3.
      References
      Product Information
      Components
      • Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial. Fifty (50) mL of Media Component.
      Quality LevelMQ100
      Applications
      ApplicationHuman H3 GPCR frozen cells for Calcium Flux FLIPR Assays.
      Key Applications
      • Calcium Flux Assays
      Biological Information
      Entrez Gene Number
      GPCR ClassA
      Host CellsChem-3
      Protein TargetH3
      Target Sub-FamilyHistamine
      Cell Line Type
      • GPCR Cell Lines
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size1 kit
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      HTS029RTA 04053252820618

      Documentation

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      Categories

      Life Science Research > Cell Culture and Systems > Cell Lines > GPCR Frozen Cells
      Life Science Research > Drug Discovery and Development > GPCR Cell Lines & Assays > GPCR Frozen Cells