western blot
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Protein Visualization | Life Science Research | Merck
Prior to probing a membrane using precious antibodies, it is helpful to visualize the transferred proteins on the blot to ensure complete transfer and even loading. This page describes possible causes and potential remedies for challenges encountered during protein visualization. -
Dot/Slot (Filtration) Blotting | Life Science Research | Merck
The possible causes and potential remedies for challenges encountered dot/slot blotting, which uses vacuum filtration to transfer protein onto a microporous membrane. -
Sample Preparation and Gel Electrophoresis | Life Science Research | Merck
The possible causes and potential remedies for challenges encountered during preparation of samples for SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) and optimizing electrophoresis conditions. -
SNAP i.d.® 2.0 System - How it Works | Life Science Research | Merck
Learn how SNAP id 2.0 protein detection system works, which applications it is compatible with, and how it optimizes western blotting conditions for reduced workflow. -
Preparing cell lysates | Life Science Research | Merck
This page describes common challenges encountered when lysing cells and extracting proteins prior to Western blotting. Total protein concentration must be determined for these cell lysates. Variables affecting each of these steps are outlined below, as each could affect the sensitivity and reproducibility of the Western blot. -
Immunodetection | Life Science Research | Merck
The possible causes and potential remedies for challenges encountered as a result of blocking, washing, antibody incubation, and detection/exposure of Western blots. -
Troubleshooting Western Blots | Life Science Research | Merck
An online troubleshooting guide describing the most common symptoms of Western blots that exhibit poor signal-to-noise, poor reproducibility, or other obstacles to interpretation. Choose a topic to view possible causes and potential remedies. -
SNAP i.d. | Merck
Merck's novel SNAP i.d.® 2.0 Protein Detection System can streamline the immunodetection reagent application phases of both Western blotting and IHC using a controlled vacuum force that removes solutions evenly - in seconds. -
SNAP i.d.® 2.0 IHC System Videos | Life Science Research | Merck
SNAP i.d.® Protein Detection System for Immunohistochemistry (IHC) is the latest capability of the SNAP i.d.® 2.0 System. It allows you to process up to 24 slides at a time with reduced handling time and variability. The system is compatible with standard IHC slides and protocols and works with diverse tissue preparations including formalin-fixed and fresh frozen samples. Watch this video for an introduction to the innovative, vacuum driven system for IHC.