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AX45005PB AXIS™ Axon Isolation Device, 450 µm, Plasma Bonded

The AXIS Axon Isolation Devices have been shown to effectively isolate cell bodies from differentiating neurites for a variety of cell types.

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Key Applications: Cell Culture
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AX45005PB
1 pack (5 devices/pack)  
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      Key Applications
      CULT
      Description
      Catalogue NumberAX45005PB
      Trade Name
      • AXIS
      DescriptionAXIS™ Axon Isolation Device, 450 µm, Plasma Bonded
      OverviewPlasma Bonded AXIS products are AXIS devices that have been permanently sealed to glass microscope slides using plasma gas exposure. This format essentially eliminates issues with leakage or cell penetration under the device and should facilitate researchers in their testing. These plasma bonded devices are currently being offered in three different formats to provide test flexibility during investigation.

      AX45005PB consists of 5 units of AX450, an axon isolation device consisting of four wells, two channels, and a set of microgrooves. Two of the wells are interconnected by a channel on each side of the device. The microgrooves are located in the area between the two channels and their length is 450µm. Each microgroove is approximately 5µm in height by 10µm in width and are designed to allow neurite passage to traverse through while preventing cell bodies from flowing across the device. There are roughly 120 microgrooves in each AX450 device.

      The AXIS Axon Isolation Devices have been shown to effectively isolate cell bodies from differentiating neurites for a variety of cell types (Taylor et al., 2005; Park et al., 2006; Liu et al., 2008; Yang, et al., 2009; Taylor et al., 2009) and will likely work with most neuronal cell type that can be successfully grown in tissue culture. Because the AXIS devices are made from an inert and non-toxic polymer, cultures grown within AXIS devices can be maintained for weeks at a time. (Chun et al., 1992; Shibayama et al., 1991). In addition, because the polymer used to fabricate the axon isolation devices is optically clear, cells cultured within the device can be imaged with high resolution microscopy, including live cell imaging, confocal, and differential interference microscopy (Taylor et al., 2005; Park et al., 2006; Liu et al., 2008; Yang, et al., 2009; Taylor et al., 2009).

      The microfluidic design of the AXIS devices is highly conducive to generation of hydrostatic pressure, which can be selectively used to isolate a solution, as well as chemical targets of interest, on a designated side of the device (Park et al., 2006). In addition, immunocytochemistry (IHC) can also be performed within an AXIS Axon Isolation device and a protocol to do so is provided in the manual.
      Alternate Names
      • AXIS™ Axon Investigation Platform
      Materials Required but Not Delivered1. Tissue culture instruments/supplies (37ºC incubator with CO2, laminar flow/tissue culture hood, growth media, flasks, etc.)
      2. Sterile 1X PBS (Millipore Cat No BSS-1005-B)
      3. Sterile Milli-Q™ water (Millipore Cat No TMS-006-B)
      4. Glass slides (25 X 75 mm) and coverslips (24 X 60 mm)
      5. Human Collagen Type II (Millipore Cat No CC052), Laminin (Millipore Cat No CC095), Poly-D-Lysine(Millipore Cat No A-003-E), or some other cell attachment solution
      6. 70% ethanol
      7. 4% paraformaldehyde
      8. Sterile plastic petri dishes (100 X 15 mm or larger)
      9. Light Diagnostic Mounting Media (Millipore Cat No 5013)
      10. DAPI (Millipore Cat No S7113)
      11. Flat Jaw Forceps (like those used for holding western blot membranes)
      12. Sonicator
      Background InformationThe development of the vertebrate nervous system entails the generation and differentiation of neurons, establishment of signaling circuits, and the formation of numerous synapses. In particular, the formation of axons and dendrites during differentiation has been an area of intense interest. Although tremendous progress has been made in this area, neuronal outgrowth studies have been hampered by the absence of a solid, easy to use, neurite culturing platform which physically orients the cells during investigation. Traditional outgrowth experiments, conducted either by culturing neural stem cells, primary neurons, or tissue slices, typically result in random, overlapping neurite extensions. The ability to address questions surrounding the specific roles of axons, dendrites and somas in neuron differentiation and growth requires a faster, more specialized method that allows scientists to spatially isolate and study individual neurites and their function. To this end, Millipore now offers its most advanced tool for the isolation, visualization, and characterization of neurite outgrowth, the AXon Investigation System (AXIS™). AXIS™ is a unique, easy to use, slide-mounted microfluidic platform that allows culture, regulation, and directional differentiation of neuronal cells via a system of growth chambers and interconnected channels. AXIS devices physically isolate developing neurites from each other and their respective neural cell bodies. In addition, the use of hydrostatic pressure during analysis provides researchers with an opportunity for targeted exposure of neuronal parts to growth factors, drug compounds, or other reagents of biological interest. This ability to selectively control exposure of neuronal outgrowth makes AXIS a powerful platform for any researcher interested in understanding axons, dendrites, somas, and their roles in synaptic formation, neural cell development, differentiation, regeneration, degeneration, and trauma.
      References
      Product Information
      Components
      • 5 devices of AX450 Plasma bonded to slides
      Quality LevelMQ100
      Applications
      ApplicationThe AXIS Axon Isolation Devices have been shown to effectively isolate cell bodies from differentiating neurites for a variety of cell types.
      Key Applications
      • Cell Culture
      Biological Information
      Cell Line Type
      • Neural Lineage Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEach unit is visually inspected to conform with internal specifications for channel width, length, and height.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsThe AXIS devices should be stored at room temperature in a clean dry area away from any chemicals or direct light. When stored properly the devices are stable for 6 months from date of receipt. Discard any remaining reagents after the expiration date.
      Packaging Information
      Material Size1 pack (5 devices/pack)
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      AX45005PB 04053252375903