217605 Anti-CD54 Mouse mAb (15.2)

217605
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      Aperçu

      Replacement Information

      Tableau de caractéristiques principal

      Species ReactivityHostAntibody Type
      H M Monoclonal Antibody

      Prix & Disponibilité

      Référence DisponibilitéConditionnement Qté Prix Quantité
      217605-100UG
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      Disponibilité limitéeDisponibilité limitée
      En stock 
      Interrompu(e)
      Disponible en quantités limitées
      Disponibilité à confirmer
        Pour le restant : Nous vous tiendrons informé
          Pour le restant : Nous vous tiendrons informé
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          Ampoule plast. 100 μg
          Prix en cours de récupération
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          Description
          OverviewRecognizes D1 domain of the ~90 kDa CD54 protein (ICAM-1) in Raji cells.
          Catalogue Number217605
          Brand Family Calbiochem®
          SynonymsAnti-ICAM-1, Domain 1
          References
          ReferencesReilly, P.L., et al. 1995. J. Immunol. 155, 529.
          Berendt, A.R., et al. 1992. Cell 68, 71.
          Dransfield, I., et al. 1992. J. Cell Biol. 116, 1527.
          Product Information
          FormLiquid
          FormulationIn 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
          Positive controlRaji cells
          Preservative0.5 mg/ml gentamicin sulfate
          Quality LevelMQ100
          Applications
          Key Applications Immunoprecipitation
          Neutralization Studies
          Flow Cytometry
          Frozen Sections
          Immunoblotting (Western Blotting)
          Application NotesFlow Cytometry (1-10 µg/ml)
          Frozen Sections (see comments)
          Immunoblotting (see comments)
          Immunoprecipitation (see comments)
          Neutralization Studies (see comments)
          Application CommentsFACS was performed on 5x105 cultured Raji human tumor cells using 1 µg/ml Anti-CD54 and FITC-conjugated goat anti-mouse IgG. This antibody will inhibit CD54 binding to LFA-1. This antibody has also been reported to work for frozen sections, immunoblotting, and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          Immunogenhuman monocytes
          ImmunogenHuman
          Clone15.2
          HostMouse
          Purity≥95% by SDS-PAGE
          IsotypeIgG₁
          Species Reactivity
          • Human
          Antibody TypeMonoclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-CD54 Mouse mAb (15.2) FDS

          Titre

          Fiche de données de sécurité des matériaux (FDS) 

          Anti-CD54 Mouse mAb (15.2) Certificats d'analyse

          TitreNuméro de lot
          217605

          Références bibliographiques

          Aperçu de la référence bibliographique
          Reilly, P.L., et al. 1995. J. Immunol. 155, 529.
          Berendt, A.R., et al. 1992. Cell 68, 71.
          Dransfield, I., et al. 1992. J. Cell Biol. 116, 1527.
          Fiche technique

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision25-July-2007 RFH
          SynonymsAnti-ICAM-1, Domain 1
          ApplicationFlow Cytometry (1-10 µg/ml)
          Frozen Sections (see comments)
          Immunoblotting (see comments)
          Immunoprecipitation (see comments)
          Neutralization Studies (see comments)
          DescriptionProtein A purified mouse monoclonal antibody. Recognizes D1 domain of the ~90 kDa CD54 protein (ICAM-1).
          BackgroundHuman CD54 (Intercellular Adhesion Molecule-1, or ICAM-1) is a ~90 kDa lymphocyte/monocyte cell surface glycoprotein that mediates cell-cell adhesion by interacting with the integrins LFA-1 (CD11a/CD18) and MAC-1 (CD11b/CD18).
          HostMouse
          Immunogen speciesHuman
          Immunogenhuman monocytes
          Clone15.2
          IsotypeIgG₁
          Specieshuman
          Positive controlRaji cells
          FormLiquid
          FormulationIn 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
          Purity≥95% by SDS-PAGE
          Preservative0.5 mg/ml gentamicin sulfate
          CommentsFACS was performed on 5x105 cultured Raji human tumor cells using 1 µg/ml Anti-CD54 and FITC-conjugated goat anti-mouse IgG. This antibody will inhibit CD54 binding to LFA-1. This antibody has also been reported to work for frozen sections, immunoblotting, and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
          Storage +2°C to +8°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
          Toxicity Standard Handling
          ReferencesReilly, P.L., et al. 1995. J. Immunol. 155, 529.
          Berendt, A.R., et al. 1992. Cell 68, 71.
          Dransfield, I., et al. 1992. J. Cell Biol. 116, 1527.