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MABC1606-100UG Anti-Caspase-8 Antibody, clone 3B10

Anti-Caspase-8, clone 3B10, Cat. No. MABC1606, is a rat monoclonal antibody that detects Caspase-8 and has been tested for use in Flow Cytometry and Western Blotting.

Anti-Caspase-8 Antibody, clone 3B10 : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
Research Category: Apoptosis & Cancer Gene Symbol: Casp8 UniProt Number: O89110
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MABC1606-100UG
100 μg  
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      Replacement Information

      Tableau de caractéristiques principal

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MWB, FCRPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABC1606-100UG
      DescriptionAnti-Caspase-8 Antibody, clone 3B10
      Alternate Names
      • EC: 3.4.22.61
      • CASP-8
      • MCH5
      • FLICE
      • MACH
      Background InformationCaspase-8 (UniProt: O89110; also known as EC: 3.4.22.61, CASP-8, CASP8, MCH5, FLICE, MACH) is encoded by the Casp8 gene (Gene ID: 12370) in murine species. Caspase-8 is a cysteine protease that plays a major role in the extrinsic apoptotic signaling pathway via death receptors. In its inactive form it associates with FADD by interactions between their DED motifs and within the FAS-FADD-Caspase 8 complex caspase 8 undergoes self-cleavage to convert to the active form. Activated caspase 8 is subsequently released to the cytosol and it cleaves downstream effector caspases 3, 4, 6, 7, 9 and 10. Caspase 8 is expressed in wide variety of tissues and its highest expression has been reported in spleen, thymus, lung, liver, and kidney. Low levels of expression are observed in heart, brain, testis, and skeletal muscle. Caspase-8 is produced with propetides (aa 1-218 and 377-387) that are cleaved under appropriate signals to produce a p20 (aa 219-376) and a p10 (aa 388-480) subunit. Active caspase 8 is a heterotetramer that consists of two p20 and two p10 subunits.
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgG1 in PBS with 0.02% sodium azide.
      Applications
      ApplicationAnti-Caspase-8, clone 3B10, Cat. No. MABC1606, is a rat monoclonal antibody that detects Caspase-8 and has been tested for use in Flow Cytometry and Western Blotting.
      Key Applications
      • Western Blotting
      • Flow Cytometry
      Application NotesFlow Cytometry Analysis: 1 µg from a representative lot detected Caspase-8 in one million mouse embryonic fibroblasts (MEF-1).
      Biological Information
      ImmunogenRecombinant p20 subunit of Mouse Caspase-8.
      Clone3B10
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      SpecificityThis rat monoclonal antibody detects Caspase-8 in murine species. It targets an epitope within the p20 subunit.
      IsotypeIgG1κ
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Casp8
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~55 kDa observed; 55.36 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse embryonic fibroblast (MEF-1) cell lysates.

      Western Blotting Analysis: 1 µg/mL of this antibody detected Caspase-8 in mouse embryonic fibroblast (MEF-1) cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      MABC1606-100UG 04054839460258