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MABF2008-100UG Anti-IL-17F Antibody, clone MM17F-8F5

Anti-IL-17F, clone MM17F-8F5, Cat. No. MABF2008, is a mouse monoclonal antibody that detects Interleukin-17F (IL-17F) and is tested for use in ELISA, Flow Cytometry, Neutralizing, and Western Blotting.

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MABF2008-100UG
100 μg  
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      Replacement Information
      Description
      Catalogue NumberMABF2008-100UG
      DescriptionAnti-IL-17F Antibody, clone MM17F-8F5
      Alternate Names
      • Interleukin-17F
      Background InformationInterleukin-17F (UniProt: Q7TNI7; also known as IL-17F) is encoded by the Il17f gene (Gene ID: 257630) in murine species. The IL-17 family of cytokines contains six members that share four conserved cysteine residues at their C-terminal region that participate in the formation of intermolecular disulfide linkages. Members of this family are associated with the pathogenesis of multiple autoimmune diseases including rheumatoid arthritis, multiple sclerosis, and inflammatory bowel diseases. IL-17F is an effector cytokine of innate and adaptive immune system involved in antimicrobial host defense and maintenance of tissue integrity. It is a disulfide-linked homodimeric protein that is synthesized with a signal peptide (aa 1-28), which is subsequently cleaved off to produce mature secreted protein (aa 29-161). It can also form a heterodimer with IL-17A. Its actions are mediated via interaction with IL-17RA and IL-17RC receptors with 2:1 binding stoichiometry. It is primarily involved in host defense against extracellular bacteria and fungi by inducing neutrophilic inflammation. As signature effector cytokine of T-helper 17 cells (Th17), it induces neutrophil activation and recruitment at infection and inflammatory sites. It is also known to stimulate the production of antimicrobial β-defensins by mucosal epithelial cells thereby limiting the entry of microbes through the epithelial barriers. (Ref.: St. Leger, AJ., et al. (2017). Immunity 47(1); 148 158; Chang, SH., and Dong, C. (2009). Cytokine.46(1); 7-11).
      References
      Product Information
      FormatPurified
      Control
      • MilliporeSigma offers a comprehensive range of trusted and highly cited primary and secondary antibodies for your research needs. Explore our 94
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      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-IL-17F, clone MM17F-8F5, Cat. No. MABF2008, is a mouse monoclonal antibody that detects Interleukin-17F (IL-17F) and is tested for use in ELISA, Flow Cytometry, Neutralizing, and Western Blotting.
      Key Applications
      • Western Blotting
      • Neutralizing
      • Flow Cytometry
      • ELISA
      Application NotesTested applications

      Flow Cytometry Analysis: A representative lot detected Interleukin-17F in Flow Cytometry application (St. Leger, A.J., et al. (2017). Immunity. 47(1):148-158.e5).

      ELISA Analysis: Various dilutions of this antibody detected recombinant mouse IL-17F.

      Neutralizing: A representative lot neutralized IL-17F activity and reduced neutrophil chemotactic activity produced by IL-17A / splenocytes activated under Th17 conditions.(Uyttenhove, C., et al. (2011). J Leukoc Biol. 89(6):1001-7).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenOvalbumin-conjugated, full-length recombinant murine IL-17F.
      EpitopeUnknown
      CloneMM17F-8F5
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone MM17F-8F5 is a mouse monoclonal antibody that detects Interleukin-17F.
      IsotypeIgG1κ
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Il17f
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~15 kDa observed; 17.94 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting with recombinant mouse IL-17F.

      Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected recombinant mouse IL-17F.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      MABF2008-100UG 04065269049210