IGF-I enhances ?5?1 integrin expression and cell motility in human chondrosarcoma cells.
Chi-Ming Wu,Te-Mao Li,Sheng-Feng Hsu,Yi-Chang Su,Shung-Te Kao,Yi-Chin Fong,Chih-Hsin Tang
Journal of cellular physiology
226
2010
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Chondrosarcoma is a type of highly malignant tumor with a potent capacity to invade locally and cause distant metastasis. Chondrosarcoma shows a predilection for metastasis to the lungs. Integrins are the major adhesive molecules in mammalian cells and have been associated with metastasis of cancer cells. Insulin-like growth factor-I (IGF)-I plays an important role in regulating cell growth, proliferation, survival, and metabolism. However, the effects of IGF-I in migration and integrin expression in chondrosarcoma cells are largely unknown. In this study, we found that IGF-I increased the migration and the expression of ?5?1 integrin in human chondrosarcoma cells. Pretreatment of cells with IGF-I receptor antibody reduced IGF-I-induced cell migration and integrin expression. Activations of phosphatidylinositol 3-kinase (PI3K), Akt, and nuclear factor-?B (NF-?B) pathways after IGF-I treatment were demonstrated, and IGF-I-induced expression of integrin and migration activity was inhibited by the specific inhibitor and mutant of PI3K, Akt, and NF-?B cascades. Taken together, our results indicated that IGF-I enhances the migration of chondrosarcoma cells by increasing ?5?1 integrin expression through the IGF-I receptor/PI3K/Akt/NF-?B signal transduction pathway. | 21344385
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