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MABC1743-25UL Anti-KCNK9 Antibody, clone Y4

Anti-KCNK9, clone Y4, Cat. No. MABC1743, is a mouse monoclonal antibody that detects Potassium channel subfamily K member 9 and is tested for use in Flow Cytometry, Function Analysis, Inhibition Assay, and Western Blotting.

MABC1743-25UL
25 µL  
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      Replacement Information
      Description
      Catalogue NumberMABC1743-25UL
      DescriptionAnti-KCNK9 Antibody, clone Y4
      Alternate Names
      • Potassium channel subfamily K member 9
      • Acid-sensitive potassium channel protein TASK-3
      • TWIK-related acid-sensitive K(+) channel 3
      • Two pore potassium channel KT3.2
      • Two pore K(+) channel KT3.2
      Background InformationPotassium channel subfamily K member 9 (UniProt: Q9NPC2; also known as Acid-sensitive potassium channel protein TASK-3, TWIK-related acid-sensitive K(+) channel 3, Two pore potassium channel KT3.2, Two pore K(+) channel KT3.2, KCNK9) is encoded by the KCNK9 (also known as TASK3) gene (Gene ID: 51305) in human. KCNK9 is a homodimeric multi-pass membrane protein of the two-pore domain potassium channel that maintains resting membrane potential of the cell. It is highly sensitive to changes in pH. It can also form heterodimer with KCNK1. It is found mainly in cerebellum, but has also been detected in adrenal gland, kidney, and lung. Over-expression of KCNK9 has been reported in several carcinomas, including breast, lung, colon, and prostate. It is reported to confer resistance to growth factor deprivation and hypoxia, which promotes tumor cell survival in hypoxic areas of solid tumors. KCNK9 has three cytoplasmic domains (aa 1-8, 129-158, and 240-374), four transmembrane domains, four extracellular domains, and two pore-forming domains (aa 89-101 and 195-207). Clone Y4 displays highest affinity for KCNK9 (KD = 0.7 nM) and induces channel internalization. Addition of Y4 to KCNK9-expressing carcinoma cells is shown to reduce their viability and increase cell death. It also inhibits the growth of human lung cancer xenografts and murine breast cancer metastasis in mice. (Ref.: Sun, H., et al. (2016). Nat. Commun. 7; 10339).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in PBS without azide
      Quality LevelMQ200
      Applications
      ApplicationAnti-KCNK9, clone Y4, Cat. No. MABC1743, is a mouse monoclonal antibody that detects Potassium channel subfamily K member 9 and is tested for use in Flow Cytometry, Function Analysis, Inhibition Assay, and Western Blotting.
      Key Applications
      • Flow Cytometry
      • Function Assay
      • Inhibition
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected KCNK9 in Western Blotting applications (Sun, H., et. al. (2016). Nat Commun. 7:10339).

      Flow Cytometry Analysis: 10 ug per million cells from a representative lot detected KCNK9 in BEN cells (Courtesy of John Laterra, M.D., Ph.D., Kennedy Krieger Institute, John Hopkins Hospital, Baltimore, MD, USA).

      Inhibits Activity/Function: A representative lot inhibited growth of human lung cancer xenografts and murine breast cancer metastasis in mice. (Sun, H., et. al. (2016). Nat Commun. 7:10339).

      Flow Cytometry Analysis: A representative lot detected KCNK9 in Flow Cytometry applications (Sun, H., et. al. (2016). Nat Commun. 7:10339).

      Affects Function: A representative lot induced endocytosis of functional channels. (Sun, H., et. al. (2016). Nat Commun. 7:10339).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenRecombinant fragment corresponding to the 59 amino acids from the first extracellular domain (M1P1 loop) of human Potassium channel subfamily K member 9 (KCNK9).
      Epitopeextracellular domain
      CloneY4
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone Y4 is a mouse monoclonal antibody that detects human Potassium channel subfamily K member 9 (KCNK9). It targets an epitope within 59 amino acids from the first extracellular loop.
      IsotypeIgG1
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • KCNK9
      • TASK3
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight42.26 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry in BT-549 cells.

      Flow Cytometry Analysis: A 1:50 dilution of this antibody detected KCNK9 in one million BT-549 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -10°C to -25°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      MABC1743-25UL 04061842414550

      Documentation

      Anti-KCNK9 Antibody, clone Y4 FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      Anti-KCNK9 Antibody, clone Y4 Certificats d'analyse

      TitreNuméro de lot
      Anti-KCNK9, clone Y4 - Q3495043 Q3495043

      Références bibliographiques

      Aperçu de la référence bibliographiqueNº PubMed
      A monoclonal antibody against KCNK9 K(+) channel extracellular domain inhibits tumour growth and metastasis
      Han Sun 1 2 , Liqun Luo 3 , Bachchu Lal 2 , Xinrong Ma 4 , Lieping Chen 5 , Christine L Hann 6 , Amy M Fulton 4 7 , Daniel J Leahy 8 , John Laterra 1 2 6 9 , Min Li
      Nat Commun  7  10339  2015

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