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MABF3284-25UG Anti-MERVL-Gag Antibody, clone A2

Anti-MERVL-Gag, clone A2, Cat. No. MABF3284, is a mouse monoclonal antibody that detects MERVL-Gag and is tested for use in Western Blotting.

MABF3284-25UG
25 μg  
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      Replacement Information
      Description
      Catalogue NumberMABF3284-25UG
      DescriptionAnti-MERVL-Gag Antibody, clone A2
      Alternate Names
      • Gag polyprotein
      Background InformationZygotic genome activation (ZGA) is a critical postfertilization step that promotes totipotency and allows different cell fates to emerge in the developing embryo. Dux (also known as Duxf3) in mouse and its human homologue DUX4 are double-homeodomain genes that are activated at the onset of ZGA in early embryos. Recent studies in 2-cell like embryonic stem cells (2C-like cells) suggest that transcription factors of the DUX family are key regulators of ZGA in placental mammals. MERVL (murine endogenous retrovirus-L) is transiently upregulated at the two-cell stage during ZGA and after the 2-cell stage, the expression of MERVL-retroelement is silenced and It has been found that this regulated pattern of MERVL expression overlapped with more than one hundred 2C-specific genes that have co-opted regulatory elements from these foreign retroviruses to begin their transcription. Although, MERVL expression is widely used as a marker of totipotency, the role of this retrotransposon in mouse embryogenesis remains elusive. Studies show that full-length MERVL transcripts, but not encoded retroviral proteins, are essential for accurate regulation of the host transcriptome and chromatin state during preimplantation development. Both knockdown and CRISPRi-based repression of MERVL result in embryonic lethality due to defects in differentiation and genomic stability. Furthermore, transcriptome and epigenome analysis has revealed that loss of MERVL transcripts led to retention of an accessible chromatin state at, and aberrant expression of, a subset of two-cell-specific genes. Gag or group-specific antigen is a polyprotein (UniProt: V9H130) and is encoded by the gag gene in mouse. It contains the core structural proteins of an Ortervirus and is believed to be antigenic. Studies suggest that this endogenous retrovirus plays a key role in regulating host cell fate potential (Ref.: Li, T.D., et al. (2022). Sci Adv. 8(47):eabq3806. (PMID: 36417507). ; Sakashita, A., et al. (2023). Nat Genet. 55(3):484-495.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-MERVL-Gag, clone A2, Cat. No. MABF3284, is a mouse monoclonal antibody that detects MERVL-Gag and is tested for use in Western Blotting.
      Key Applications
      • Western Blotting
      Application NotesTested Applications

      Western Blotting Analysis: A representative lot detected MERVL-GAG in Western Blotting application (Li, T.D., et al. (2022). Sci Adv. 8(47):eabq3806; Sakashita, A., et al. (2023). Nat Genet. 55(3):484-495).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenGST-tagged, fragment recombinant from mouse Gag polyprotein corresponding to 200 amino acids from the N-terminal half of mouse MERVL-gag protein.
      EpitopeN-terminal half
      CloneA2
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone A2 is a mouse monoclonal antibody that specifically detects MERVL-Gag protein. It targets an epitope within 200 amino acids from the N-terminal half.
      IsotypeIgG2aκ
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • gag
      Purification MethodProtein G purified
      UniProt Number
      Molecular WeightTarget molecular weight ~ 66 kDa observed; 66.7 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in lysates from mESCs transiently over-expressing Duxf3.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected MERVL-Gag in lysates from mESCs transiently over-expressing Duxf3.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      MABF3284-25UG 04065272072861