Millipore Sigma Vibrant Logo

MABC186-I Anti-phospho-p62 (Ser403) Antibody, clone 4F6

Detect p62/SQSTM1 (Ser403) using this rat monoclonal Anti-phospho-p62 (Ser403) antibody, clone 4F6, Cat. No. MABC186-I. Validated for use in Immunohistochemistry and Western Blotting.

FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
Research Category: Apoptosis & Cancer Gene Symbol: SQSTM1, ORCA, OSIL UniProt Number: Q13501
View Products on Sigmaaldrich.com
MABC186-I
100 μg  
Prix en cours de récupération
Le prix n'a pas pu être récupéré
La quantité minimale doit être un multiple de
Maximum Quantity is
À la validation de la commande Plus d'informations
Vous avez sauvegardé ()
 
—
Demander le prix
—
Disponibilité limitée Disponibilité limitée
En stock 
Interrompu(e)
Disponible en quantités limitées
Disponibilité à confirmer
    Pour le restant : Nous vous tiendrons informé
      Pour le restant : Nous vous tiendrons informé
      Nous vous tiendrons informé
      Contacter le Service Clients
      Contact Customer Service

       

      Contacter le Service Clients

      Aperçu

      Replacement Information

      Tableau de caractéristiques principal

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, HWB, IHCRPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABC186-I
      DescriptionAnti-phospho-p62 (Ser403) Antibody, clone 4F6
      Alternate Names
      • Ubiquitin-binding protein p62
      • p62
      • EBI3-associated protein of 60 kDa
      • EBIAP
      • p60
      • Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa
      Background InformationSequestosome-1 (UniProt: Q13501; also known as EBI3-associated protein of 60 kDa; EBIAP; p60; Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa; Ubiquitin-binding protein p62) is encoded by the SQSTM1 (also known as ORCA, OSIL) gene (Gene ID 8878) in human Sequestosome is a multi-functional co-interacting protein with a UBA domain at its C-terminal end, which binds non-covalently to polyubiquitin chains and regulates the activation of the nuclear factor kappa-B (NF-kappaB) signaling. It functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 (TRAF6) to mediate the activation of NF- B in response to upstream signals. It also serves as an autophagosome cargo protein that targets other proteins that bind to it for selective autophagy. Defects in sequestosome-1 are a cause of Paget disease of bone (PDB), a metabolic bone disease affecting the axial skeleton and is characterized by focal areas of increased and disorganized bone turn-over due to activated osteoclasts. Mutations in SQSTM1 gene can lead to frontotemporal dementia and/or amyotrophic lateral sclerosis 3. Frontotemporal dementia is characterized by frontal and temporal lobe atrophy associated with neuronal loss, gliosis, and dementia. Amyotrophic lateral sclerosis is characterized by the death of motor neurons in the brain, brainstem, and spinal cord, resulting in fatal paralysis.
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect p62/SQSTM1 (Ser403) using this rat monoclonal Anti-phospho-p62 (Ser403) antibody, clone 4F6, Cat. No. MABC186-I. Validated for use in Immunohistochemistry and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesWestern Blotting Analysis: A representative lot detected p62/SQSTM1 (Ser403) in a Western Blot application. (Matsumoto, G., et. al. (2011). Mol Cell. 44(2):279-289).

      Immunohistochemistry Analysis: A representative lot detected p62/SQSTM1 (Ser403) in a Immunohistochemistry application. (Matsumoto, G., et. al. (2011). Mol Cell. 44(2):279-289).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to a sequence surrounding pSer403 of human phospho p62/SQSTM1.
      Clone4F6
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      SpecificityClone 4F6 recognizes p62/SQSTM1 phosphorylated at Ser403 in human and mouse.
      IsotypeIgG2aκ
      Species Reactivity
      • Mouse
      • Human
      Species Reactivity NoteHuman, Mouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SQSTM1
      • ORCA
      • OSIL
      Modifications
      • Phosphorylation
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~60 kDa observed; 46.68 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Neuro2a cells.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected p62/SQSTM1 (Ser403) in Neuro2a cells in which GFP fused human p62 (G-p62) was stably transfected and treated with MG132 vs. an untreated sample.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      MABC186-I 04054839155994

      Documentation

      Anti-phospho-p62 (Ser403) Antibody, clone 4F6 FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      Anti-phospho-p62 (Ser403) Antibody, clone 4F6 Certificats d'analyse

      TitreNuméro de lot
      Anti-phospho-p62 (Ser403), clone 4F6 -Q2809481 Q2809481
      Anti-phospho-p62 (Ser403), clone 4F6 Monoclonal Antibody 3152148
      Anti-phospho-p62 (Ser403), clone 4F6 Monoclonal Antibody 3067423
      Anti-phospho-p62 (Ser403), clone 4F6 Monoclonal Antibody 3009721
      Anti-phospho-p62 (Ser403), clone 4F6 Monoclonal Antibody 3033467