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216254 BCECF/AM - CAS 117464-70-7 - Calbiochem

216254
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216254-1MG
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      Description
      OverviewA variable mixture of cell-permeable ester derivatives of BCECF that are hydrolyzed by cytosolic esterases to yield intracellularly trapped indicator BCECF. Has been used to monitor intracellular pH changes in mammalian fibroblasts, gastric cells, lymphocytes, myocytes, and distal convoluted tubules.
      Catalogue Number216254
      Brand Family Calbiochem®
      Synonyms2ʹ,7ʹ-bis-(Carboxyethyl)-5(6ʹ)-carboxyfluorescein Acetoxymethyl Ester
      References
      ReferencesFutsaetner, C.M., et al. 1993. Can. J. Microbiol. 39, 180.
      Hayashi, H., et al. 1992. Am. J. Physiol. 262, C628.
      Sasaki, S., et al. 1992. Biochim. Biophys. Acta 1137, 45.
      Miyata, H., et al. 1989. Biochem. Biophys. Res. Commun. 163, 500.
      Weiner, I.D., and Hamm, L.L. 1989. Am. J. Physiol. 256, F957.
      Product Information
      CAS number117464-70-7
      ATP CompetitiveN
      FormWhite to off-white solid
      ReversibleN
      Quality LevelMQ100
      Applications
      Biological Information
      Primary TargetMonitor intracellular pH changes
      Purity≥85% by HPLC (mixture of isomers)
      Physicochemical Information
      Cell permeableY
      Emission max.
      Excitation max.
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      R PhraseR: 36/37/38

      Irritating to eyes, respiratory system and skin.
      S PhraseS: 36-22-26

      Wear suitable protective clothing.
      Do not breathe dust.
      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Irritant
      Storage -20°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 week at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      BCECF/AM - CAS 117464-70-7 - Calbiochem FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      BCECF/AM - CAS 117464-70-7 - Calbiochem Certificats d'analyse

      TitreNuméro de lot
      216254

      Références bibliographiques

      Aperçu de la référence bibliographique
      Futsaetner, C.M., et al. 1993. Can. J. Microbiol. 39, 180.
      Hayashi, H., et al. 1992. Am. J. Physiol. 262, C628.
      Sasaki, S., et al. 1992. Biochim. Biophys. Acta 1137, 45.
      Miyata, H., et al. 1989. Biochem. Biophys. Res. Commun. 163, 500.
      Weiner, I.D., and Hamm, L.L. 1989. Am. J. Physiol. 256, F957.

      Brochure

      Titre
      Caspases and other Apoptosis Related Tools Brochure
      Fiche technique

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision06-February-2019 JSW
      Synonyms2ʹ,7ʹ-bis-(Carboxyethyl)-5(6ʹ)-carboxyfluorescein Acetoxymethyl Ester
      DescriptionA variable mixture of cell-permeable ester derivatives of BCECF (Cat. No. 216252) that are hydrolyzed by cytosolic esterases to yield intracellularly trapped indicator BCECF. Has been used to monitor intracellular pH changes in mammalian fibroblasts, gastric cells, lymphocytes, myocytes, and distal convoluted tubules. Typical loading buffer contains 1-10 µM BCECF/AM in serum-free medium that is free of amino acids and buffers containing primary or secondary amines.
      FormWhite to off-white solid
      Intert gas (Yes/No) Packaged under inert gas
      CAS number117464-70-7
      Purity≥85% by HPLC (mixture of isomers)
      SolubilityDMSO, DMF, or Acetonitrile (25 mg/ml)
      Storage -20°C
      Protect from light
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 week at -20°C.
      Toxicity Irritant
      ReferencesFutsaetner, C.M., et al. 1993. Can. J. Microbiol. 39, 180.
      Hayashi, H., et al. 1992. Am. J. Physiol. 262, C628.
      Sasaki, S., et al. 1992. Biochim. Biophys. Acta 1137, 45.
      Miyata, H., et al. 1989. Biochem. Biophys. Res. Commun. 163, 500.
      Weiner, I.D., and Hamm, L.L. 1989. Am. J. Physiol. 256, F957.