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208772 Calpain-1 Substrate II, Fluorogenic - Calbiochem

208772
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208772-1MG
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      Description
      OverviewAn internally quenched peptide substrate that is optimized for calpain-1 amino acid recognition motifs at the primed as well as the non-primed sides. Shown to be kinetically superior, in terms of turnover rate, to Calpain Substrate III, Fluorogenic (Cat. No. 208771), Calpain-1 Substrate, Fluorogenic (Cat. No. 208748), and Calpain Substrate II, Fluorogenic (Cat. No. 208731) by ~8-, 18-, and 310-fold, respectively.
      Catalogue Number208772
      Brand Family Calbiochem®
      SynonymsH-E(EDANS)PLF~AERK(DABCYL)-OH
      References
      ReferencesCuerrier, D., et al. 2007. J. Biol. Chem. 282, 9600.
      Cuerrier, D., et al. 2005. J. Biol. Chem. 280, 40632.
      Product Information
      ATP CompetitiveN
      FormRed lyophilized solid
      FormulationSupplied as a trifluoroacetate salt.
      Hill FormulaC₇₂H₉₇N₁₇O₁₆S
      Chemical formulaC₇₂H₉₇N₁₇O₁₆S
      Hygroscopic Hygroscopic
      ReversibleN
      Quality LevelMQ100
      Applications
      Biological Information
      Primary TargetCalpain-1 Substrate
      Purity≥97% by HPLC
      Physicochemical Information
      Cell permeableN
      Emission max.
      Excitation max.
      Peptide SequenceH₂N-Glu(EDANS)-Pro-Leu-Phe~Ala-Glu-Arg-Lys(DABCYL)-CO₂H
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Irritant
      Storage -20°C
      Protect from Light Protect from light
      Hygroscopic Hygroscopic
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Calpain-1 Substrate II, Fluorogenic - Calbiochem FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      Calpain-1 Substrate II, Fluorogenic - Calbiochem Certificats d'analyse

      TitreNuméro de lot
      208772

      Références bibliographiques

      Aperçu de la référence bibliographique
      Cuerrier, D., et al. 2007. J. Biol. Chem. 282, 9600.
      Cuerrier, D., et al. 2005. J. Biol. Chem. 280, 40632.
      Fiche technique

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision31-January-2008 JSW
      SynonymsH-E(EDANS)PLF~AERK(DABCYL)-OH
      DescriptionAn internally quenched peptide substrate that is optimized for calpain-1 amino acid recognition motifs at the primed as well as the non-primed sides. Shown to be kinetically superior, in terms of turnover rate, to Calpain Substrate III, Fluorogenic (Cat. No. 208771), Calpain-1 Substrate, Fluorogenic (Cat. No. 208748), and Calpain Substrate II, Fluorogenic (Cat. No. 208731) by ~8-, 18-, and 310-fold, respectively. Excitation: ~335 nm; emission: ~505 nm.
      FormRed lyophilized solid
      FormulationSupplied as a trifluoroacetate salt.
      Intert gas (Yes/No) Packaged under inert gas
      Chemical formulaC₇₂H₉₇N₁₇O₁₆S
      Peptide SequenceH₂N-Glu(EDANS)-Pro-Leu-Phe~Ala-Glu-Arg-Lys(DABCYL)-CO₂H
      Purity≥97% by HPLC
      SolubilityDMSO (5 mg/ml)
      Storage -20°C
      Hygroscopic
      Protect from light
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Toxicity Irritant
      ReferencesCuerrier, D., et al. 2007. J. Biol. Chem. 282, 9600.
      Cuerrier, D., et al. 2005. J. Biol. Chem. 280, 40632.