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219399 Cathepsin D Substrate I - Calbiochem

219399
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219399-10MG
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      Ampoule plast. 10 mg
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      Description
      OverviewUseful as a substrate for the determination of cathepsin D activity. Suitable for either colorimetric or fluorometric assay. Cathepsin D hydrolyzes the Phe-Phe bond. Requires dipeptidyl aminopeptidase II to free 4-methoxy-β-naphthylamine (4MeOβNA).
      Catalogue Number219399
      Brand Family Calbiochem®
      SynonymsBz-RGFFP-4-MeOβNA, HCl
      References
      ReferencesTakahashi, T., and Tang, J. 1981. Methods Enzymol. 80, 565.
      Smith, R.E., and Van Frank, R.M. 1975. in Lysosomes in Biology and Pathology 4 (Dingle, J.T., and Dean, R.T., eds.) p. 193, American Elsevier, Holland.
      Product Information
      ATP CompetitiveN
      FormWhite lyophilized solid
      Hill FormulaC₄₉H₅₅N₉O₇ · HCl
      Chemical formulaC₄₉H₅₅N₉O₇ · HCl
      ReversibleN
      Quality LevelMQ100
      Applications
      Biological Information
      Primary TargetSubstrate for the determination of cathepsin D activity
      Purity≥95% by HPLC
      Physicochemical Information
      Cell permeableN
      Emission max.
      Excitation max.
      Peptide SequenceBz-Arg-Gly-Phe-Phe-Pro-4MeOβNA, HCl
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Protect from Light Protect from light
      Protect from Moisture Protect from moisture
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 4 months at -20°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Cathepsin D Substrate I - Calbiochem FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      Cathepsin D Substrate I - Calbiochem Certificats d'analyse

      TitreNuméro de lot
      219399

      Références bibliographiques

      Aperçu de la référence bibliographique
      Takahashi, T., and Tang, J. 1981. Methods Enzymol. 80, 565.
      Smith, R.E., and Van Frank, R.M. 1975. in Lysosomes in Biology and Pathology 4 (Dingle, J.T., and Dean, R.T., eds.) p. 193, American Elsevier, Holland.

      Brochure

      Titre
      Cathepsins and Related Products Technical Bulletin
      Fiche technique

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision05-June-2008 RFH
      SynonymsBz-RGFFP-4-MeOβNA, HCl
      DescriptionA useful substrate for colorimetric or fluorometric assay of cathepsin D activity. Cathepsin D hydrolyzes the Phe-Phe bond. Dipeptidyl aminopeptidase II is required to free the 4-methoxy-β-naphthylamine (4MβNA). Excitation max: ~345 nm; emission max: ~425 nm.
      FormWhite lyophilized solid
      Recommended reaction conditions
      1. Incubate the substrate, Bz-Arg-Gly-Phe-Phe-Pro-4MβNA (final concentration 350 µM) with cathepsin D in 500 µl of reaction buffer (20 mM glycine-HCl, pH 3.0) at 37°C to allow digestion of the substrate to Bz-Arg-Gly-Phe-OH and Phe-Pro-4MβNA. 2. Stop the reaction by adding 50 µl of 1% KOH and then add 850 µL of reaction buffer 2 (100 mM Tris-HCl, pH 7.8). 3. Add 1 mU dipeptidylpeptidase IV in 100 µl of reaction buffer 2 and incubate for >1 h at 37°C to completely hydrolyze the Phe-Pro-4MβNA to Phe-Pro-OH and 4MβNA. 4. Monitor the release of 4MβNA at 345 nm (excitation) and 425 nm (emission).
      Chemical formulaC₄₉H₅₅N₉O₇ · HCl
      Peptide SequenceBz-Arg-Gly-Phe-Phe-Pro-4MeOβNA, HCl
      Purity≥95% by HPLC
      SolubilityDMSO (5 mg/ml)
      Storage -20°C
      Protect from moisture
      Protect from light
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 4 months at -20°C.
      Toxicity Standard Handling
      ReferencesTakahashi, T., and Tang, J. 1981. Methods Enzymol. 80, 565.
      Smith, R.E., and Van Frank, R.M. 1975. in Lysosomes in Biology and Pathology 4 (Dingle, J.T., and Dean, R.T., eds.) p. 193, American Elsevier, Holland.