220477 Chitosanase, Streptomyces sp. N174

220477
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220477-10U
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      Description
      OverviewNative Chitosanase from Streptomyces species. A member of a new class of enzymes with antifungal properties that efficiently catalyzes the hydrolysis of acetylated chitosan (0 to 60%). Does not hydrolyze chitin or carboxylmethylcellulose. Acts by splitting the β1,4-glycosidic linkages of GlcNAc-GlcN (Km = 29.3 µg/ml) and GlcNAc-GlcN. Its activity generally increases with decreasing acetylation of chitosan. Suitable for the efficient preparation of protoplasts of Mucor and other Zygomycetes, allowing the genetic manipulation of these fungi.
      Catalogue Number220477
      Brand Family Calbiochem®
      SynonymsChitosan N-acetylglucosaminohydrolase
      References
      ReferencesBoucher, I., et al. 1995. J. Biol. Chem. 270, 31077.
      Cuero, R.G., and Osuji, G.O. 1995. Food Addit. Contam. 12, 479.
      Fukamizo, T., et al. 1995. Biochem J. 311, 377.
      Li, T., et al. 1995. Plant Physiol. Biochem. 33, 599.
      Fukamizo, T., et al. 1994. Biochim. Biophys. Acta 1205, 183.
      Osswald, W.F., et al. 1994. Plant Cell Physiol. 35, 811.
      Product Information
      CAS number51570-20-8
      Unit of DefinitionOne unit is defined as the amount of enzyme that will release 1.0 µmol reducing sugars or glucosamine from chitosan per min at 37°C, pH 5.5.
      EC number3.2.1.132
      FormLiquid
      FormulationIn 50 M sodium acetate, 50% glycerol, pH 5.0.
      PI7.5, temperature optimum 65°C for 10 minutes; recommended temperature 37°C
      Quality LevelMQ100
      Applications
      Biological Information
      Specific Activity≥25 units/mg protein
      Physicochemical Information
      Contaminantschitinolytic activity: ≤0.01%, proteases: <0.25 Azocasein U/ml (One Azocasein unit is defined as the amount of protease that will liberate sufficient pigment to increase the optical density at 366 nm by 0.01 after incubation for 30 minutes with Azocasein)
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Chitosanase, Streptomyces sp. N174 FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      Chitosanase, Streptomyces sp. N174 Certificats d'analyse

      TitreNuméro de lot
      220477

      Références bibliographiques

      Aperçu de la référence bibliographique
      Boucher, I., et al. 1995. J. Biol. Chem. 270, 31077.
      Cuero, R.G., and Osuji, G.O. 1995. Food Addit. Contam. 12, 479.
      Fukamizo, T., et al. 1995. Biochem J. 311, 377.
      Li, T., et al. 1995. Plant Physiol. Biochem. 33, 599.
      Fukamizo, T., et al. 1994. Biochim. Biophys. Acta 1205, 183.
      Osswald, W.F., et al. 1994. Plant Cell Physiol. 35, 811.
      Fiche technique

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision07-March-2019 JSW
      SynonymsChitosan N-acetylglucosaminohydrolase
      DescriptionNative Chitosanase from Streptomyces species. Chromatographically purified. Chitosanase is a member of a new class of enzymes with antifungal properties that efficiently catalyzes the hydrolysis of acetylated chitosan (0 to 60%). Does not hydrolyze chitin or carboxylmethyl cellulose. Acts by splitting the b1,4-glycosidic linkages of GlcN-GlcN (Km = 29.3 µg/ml) and GlcNAc-GlcN. Its activity generally increases with decreasing acetylation of chitosan. Suitable for the efficient preparation of protoplasts of Mucor and other Zygomycetes, allowing the genetic manipulation of these fungi. Has an optimal pH of 5.5, an optimal temperature of 65°C for 10 min, and a recommended temperature of 37°C.
      FormLiquid
      FormulationIn 50 M sodium acetate, 50% glycerol, pH 5.0.
      CAS number51570-20-8
      EC number3.2.1.132
      Contaminantschitinolytic activity: ≤0.01%, proteases: <0.25 Azocasein U/ml (One Azocasein unit is defined as the amount of protease that will liberate sufficient pigment to increase the optical density at 366 nm by 0.01 after incubation for 30 minutes with Azocasein)
      Specific activity≥25 units/mg protein
      Unit definitionOne unit is defined as the amount of enzyme that will release 1.0 µmol reducing sugars or glucosamine from chitosan per min at 37°C, pH 5.5.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesBoucher, I., et al. 1995. J. Biol. Chem. 270, 31077.
      Cuero, R.G., and Osuji, G.O. 1995. Food Addit. Contam. 12, 479.
      Fukamizo, T., et al. 1995. Biochem J. 311, 377.
      Li, T., et al. 1995. Plant Physiol. Biochem. 33, 599.
      Fukamizo, T., et al. 1994. Biochim. Biophys. Acta 1205, 183.
      Osswald, W.F., et al. 1994. Plant Cell Physiol. 35, 811.