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234109 Colcemid - CAS 477-30-5 - Calbiochem

234109
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Tableau de caractéristiques principal

CAS #Empirical Formula
477-30-5C₂₁H₂₅NO₅

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234109-5MG
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      Description
      OverviewCell synchronization agent. Induces apoptosis by blocking mitosis in HeLa S3 cells. Depolymerizes microtubules and limits microtubule formation. Appropriate dilutions inactivate spindle fiber mechanism during metaphase, systematically immobilizing chromosomes at the metaphase plate.
      Catalogue Number234109
      Brand Family Calbiochem®
      SynonymsDemecolcine
      References
      ReferencesUrbani, L., et al. 1995. Exp. Cell Res. 219, 159.
      Kanda, R., et al. 1994. J. Radiat. Res. 35, 41.
      Sherwood, S.W., et al. 1994. Exp. Cell Res. 215, 373.
      Nishiyama, I., and Fuji, T. 1992. Exp. Cell Res. 198, 214.
      Spoerri, P.E., and Roisen, F.J. 1992. J. Neurosci. Res. 31, 494.
      Product Information
      CAS number477-30-5
      ATP CompetitiveN
      FormCrystalline powder
      Hill FormulaC₂₁H₂₅NO₅
      Chemical formulaC₂₁H₂₅NO₅
      ReversibleN
      Structure formula ImageStructure formula Image
      Quality LevelMQ100
      Applications
      Biological Information
      Primary TargetDepolymerizes microtubules and limits microtubule formation.
      Purity≥98% by HPLC
      Physicochemical Information
      Cell permeableN
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      RTECSGH0800000
      Safety Information
      R PhraseR: 25-63

      Toxic if swallowed.
      Possible risk of harm to the unborn child.
      S PhraseS: 22-36/37/39-45

      Do not breathe dust.
      Wear suitable protective clothing, gloves and eye/face protection.
      In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Toxic & Carcinogenic / Teratogenic
      Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
      Storage +15°C to +30°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) under an inert gas. Stock solutions are stable for up to 6 months at -20°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Colcemid - CAS 477-30-5 - Calbiochem FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      Colcemid - CAS 477-30-5 - Calbiochem Certificats d'analyse

      TitreNuméro de lot
      234109

      Références bibliographiques

      Aperçu de la référence bibliographique
      Urbani, L., et al. 1995. Exp. Cell Res. 219, 159.
      Kanda, R., et al. 1994. J. Radiat. Res. 35, 41.
      Sherwood, S.W., et al. 1994. Exp. Cell Res. 215, 373.
      Nishiyama, I., and Fuji, T. 1992. Exp. Cell Res. 198, 214.
      Spoerri, P.E., and Roisen, F.J. 1992. J. Neurosci. Res. 31, 494.
      Fiche technique

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision03-April-2008 RFH
      SynonymsDemecolcine
      DescriptionCell synchronization agent. Induces apoptosis by blocking mitosis in HeLa S3 cells. Depolymerizes microtubules and limits microtubule formation. Appropriate dilutions inactivate the spindle fiber mechanism during metaphase, systematically immobilizing chromosomes at the metaphase plate.
      FormCrystalline powder
      CAS number477-30-5
      RTECSGH0800000
      Chemical formulaC₂₁H₂₅NO₅
      Structure formulaStructure formula
      Purity≥98% by HPLC
      SolubilityEthanol and chloroform (10 mg/ml)
      Storage +15°C to +30°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) under an inert gas. Stock solutions are stable for up to 6 months at -20°C.
      Toxicity Toxic & Carcinogenic / Teratogenic
      Merck USA index14, 2887
      ReferencesUrbani, L., et al. 1995. Exp. Cell Res. 219, 159.
      Kanda, R., et al. 1994. J. Radiat. Res. 35, 41.
      Sherwood, S.W., et al. 1994. Exp. Cell Res. 215, 373.
      Nishiyama, I., and Fuji, T. 1992. Exp. Cell Res. 198, 214.
      Spoerri, P.E., and Roisen, F.J. 1992. J. Neurosci. Res. 31, 494.