371206 Dkk-1, His•Tag®, Mouse, Recombinant

371206
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      Aperçu

      Replacement Information

      Tableau de caractéristiques principal

      Purity
      ≥95% by SDS-PAGE

      Prix & Disponibilité

      Référence DisponibilitéConditionnement Qté Prix Quantité
      371206-10UG
      Récupération des données relatives à la disponibilité...
      Disponibilité limitéeDisponibilité limitée
      En stock 
      Interrompu(e)
      Disponible en quantités limitées
      Disponibilité à confirmer
        Pour le restant : Nous vous tiendrons informé
          Pour le restant : Nous vous tiendrons informé
          Nous vous tiendrons informé
          Contacter le Service Clients
          Contact Customer Service

          Flacon en verre 10 μg
          Prix en cours de récupération
          Le prix n'a pas pu être récupéré
          La quantité minimale doit être un multiple de
          À la validation de la commande Plus d'informations
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          Description
          OverviewRecombinant, murine Dickkopf related protein-1 (Dkk-1), consisting of amino acids 30-272, fused at the C-terminus to a His•Tag® sequence, and expressed in NS0-dervied mouse myeloma cells. Dkk-1 is a well-documented antagonist of the Wnt signaling pathway. The balance between Dkk-1 inhibition and Wnt signaling is essential for bone formation and homeostasis.
          Catalogue Number371206
          Brand Family Calbiochem®
          References
          ReferencesPinzone, J.J. et al. 2009. Blood 113, 517.
          Li, J. et al. 2006. Bone 36, 754.
          Morvan, F. et al. 2006. J. Bone Miner. Res. 21, 934.
          Niehrs, C. 2006. Oncogene 25, 7469.
          Verani, R. et al. 2006. J. Neurochem. 101, 242.
          Cappuccio, I. et al. 2005. J. Neurosci. 25, 2647.
          Kemp, C. et al. 2005. Dev. Dyn. 233, 1064.
          Adamska, M. et al. 2004. Dev. Biol. 272, 134.
          Bullock, C.M. et al. 2004. Mol. Pharmacol. 65, 582.
          Mao, B. et al. 2002. Nature 417, 664.
          Mao, B. et al. 2001. Nature 411, 321.
          Glinka, A. et al. 1998. Nature 391, 357.
          Product Information
          FormLyophilized
          FormulationLyophilized from 0.2 µm filtered PBS, BSA.
          Quality LevelMQ100
          Applications
          Biological Information
          Biological activityND₅₀: 0.2-1 µg/ml as measured by the inhibition of Wnt-3a-induced alkaline phosphatase production in MC3T3-E1 mouse osteoblastic cells.
          Reduction of Axis-inducing activity: 60-100% reduction (using 1 ng rmDkk-1 per embryo) in the number of secondary axes produced in the presence of 10-20 pg Xwnt-8 mRNA using early Xenopus embryos.
          Purity≥95% by SDS-PAGE
          Physicochemical Information
          ContaminantsEndotoxin: ≤1.0 EU/µg protein
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Regulatory Review
          Storage -20°C
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Dkk-1, His•Tag®, Mouse, Recombinant FDS

          Titre

          Fiche de données de sécurité des matériaux (FDS) 

          Dkk-1, His•Tag®, Mouse, Recombinant Certificats d'analyse

          TitreNuméro de lot
          371206

          Références bibliographiques

          Aperçu de la référence bibliographique
          Pinzone, J.J. et al. 2009. Blood 113, 517.
          Li, J. et al. 2006. Bone 36, 754.
          Morvan, F. et al. 2006. J. Bone Miner. Res. 21, 934.
          Niehrs, C. 2006. Oncogene 25, 7469.
          Verani, R. et al. 2006. J. Neurochem. 101, 242.
          Cappuccio, I. et al. 2005. J. Neurosci. 25, 2647.
          Kemp, C. et al. 2005. Dev. Dyn. 233, 1064.
          Adamska, M. et al. 2004. Dev. Biol. 272, 134.
          Bullock, C.M. et al. 2004. Mol. Pharmacol. 65, 582.
          Mao, B. et al. 2002. Nature 417, 664.
          Mao, B. et al. 2001. Nature 411, 321.
          Glinka, A. et al. 1998. Nature 391, 357.
          Fiche technique

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision22-June-2011 RFH
          DescriptionRecombinant, murine Dickkopf related protein-1 (Dkk-1), consisting of amino acids 30-272, fused at the C-terminus to a His•Tag® sequence, and expressed in NS0-dervied mouse myeloma cells. Dkk-1 is a well-documented antagonist of the Wnt signaling pathway. The balance between Dkk-1 inhibition and Wnt signaling is essential for bone formation and homeostasis.
          FormLyophilized
          FormulationLyophilized from 0.2 µm filtered PBS, BSA.
          Purity≥95% by SDS-PAGE
          ContaminantsEndotoxin: ≤1.0 EU/µg protein
          Biological activityND₅₀: 0.2-1 µg/ml as measured by the inhibition of Wnt-3a-induced alkaline phosphatase production in MC3T3-E1 mouse osteoblastic cells.
          Reduction of Axis-inducing activity: 60-100% reduction (using 1 ng rmDkk-1 per embryo) in the number of secondary axes produced in the presence of 10-20 pg Xwnt-8 mRNA using early Xenopus embryos.
          SolubilityReconstitute to 100 µg/ml with sterile PBS containing ≥1% human or bovine serum albumin.
          Storage -20°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
          Toxicity Regulatory Review
          ReferencesPinzone, J.J. et al. 2009. Blood 113, 517.
          Li, J. et al. 2006. Bone 36, 754.
          Morvan, F. et al. 2006. J. Bone Miner. Res. 21, 934.
          Niehrs, C. 2006. Oncogene 25, 7469.
          Verani, R. et al. 2006. J. Neurochem. 101, 242.
          Cappuccio, I. et al. 2005. J. Neurosci. 25, 2647.
          Kemp, C. et al. 2005. Dev. Dyn. 233, 1064.
          Adamska, M. et al. 2004. Dev. Biol. 272, 134.
          Bullock, C.M. et al. 2004. Mol. Pharmacol. 65, 582.
          Mao, B. et al. 2002. Nature 417, 664.
          Mao, B. et al. 2001. Nature 411, 321.
          Glinka, A. et al. 1998. Nature 391, 357.