324715 Endoproteinase Lys-C, Sequencing Grade, Lysobacter enzymogenes

324715
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      Description
      OverviewNative endoproteinase Lys-C from Lysobacter enzymogenes. Serine protease that specifically hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine in peptides and proteins. Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Inhibited by aprotinin, DFP, leupeptin, and TLCK. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.
      Catalogue Number324715
      Brand Family Calbiochem®
      References
      ReferencesJekel, P.A., et al. 1983. Anal. Biochem. 134, 347.
      Product Information
      CAS number72561-05-8
      Unit of DefinitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol Tos-Gly-Pro-Lys-<i>p</i>NA per min at 25°C, pH 7.7.
      EC number3.4.21.50
      FormLyophilized
      FormulationLyophilized from 50 mM HEPES, 10 mM EDTA, 5 mg/ml raffinose, pH 8.0.
      Hygroscopic Hygroscopic
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥90% by SDS-PAGE
      Specific Activity≥200 units/mg protein
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      R PhraseR: 20-36/37/38-42/43

      Harmful by inhalation.
      Irritating to eyes, respiratory system and skin.
      May cause sensitization by inhalation and skin contact.
      S PhraseS: 22-26-36

      Do not breathe dust.
      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Wear suitable protective clothing.
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Harmful
      Storage +2°C to +8°C
      Hygroscopic Hygroscopic
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 2 days at 4°C or for up to 1 month at -20°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Endoproteinase Lys-C, Sequencing Grade, Lysobacter enzymogenes FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      Endoproteinase Lys-C, Sequencing Grade, Lysobacter enzymogenes Certificats d'analyse

      TitreNuméro de lot
      324715

      Références bibliographiques

      Aperçu de la référence bibliographique
      Jekel, P.A., et al. 1983. Anal. Biochem. 134, 347.
      Fiche technique

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision01-February-2012 RFH
      DescriptionNative endoproteinase Lys-C from Lysobacter enzymogenes. Serine protease that specifically hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine in peptides and proteins. Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Inhibited by aprotinin, DFP, leupeptin, and TLCK.
      FormLyophilized
      FormulationLyophilized from 50 mM HEPES, 10 mM EDTA, 5 mg/ml raffinose, pH 8.0.
      Recommended reaction conditions
      Protocol 1. Dissolve the proteins to be sequenced in 25 mM Tris-HCl, pH 8.5, and 1 mM EDTA. For proteins that are difficult to solubilize, add urea, SDS, or guanidine-HCl to the digestion buffer prior to solubilization. If urea is used, add 20 mmol/l of methylamine. 2. The suggested working concentration is 1:20-1:100 (protease:protein by weight). 3. Incubate for 2-18 h at 37°C.
      CAS number72561-05-8
      EC number3.4.21.50
      Purity≥90% by SDS-PAGE
      Specific activity≥200 units/mg protein
      Unit definitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol Tos-Gly-Pro-Lys-pNA per min at 25°C, pH 7.7.
      SolubilityReconstitute in 50 µl of distilled H₂O.
      Storage +2°C to +8°C
      Hygroscopic
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 2 days at 4°C or for up to 1 month at -20°C.
      Toxicity Harmful
      ReferencesJekel, P.A., et al. 1983. Anal. Biochem. 134, 347.

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      Catégories

      Life Science Research > Proteins and Enzymes > Other Enzymes