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324867 PhosphoDetect™ Anti-EGFR (pTyr¹⁰⁶⁸) Rabbit pAb

324867
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Tableau de caractéristiques principal

Species ReactivityHostAntibody Type
Ch, H, M, RRbPolyclonal Antibody

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324867-10T
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      Ampoule plast. 10 t
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      Description
      OverviewRecognizes the ~185 kDa EGFR protein phosphorylated at Tyr1068. Note: 1 T = 1 test. Sufficient for 10 Western miniblots.
      Catalogue Number324867
      Brand Family Calbiochem®
      SynonymsAnti-Epidermal Growth Factor Receptor (pTyr¹⁰⁶⁸)
      Application Data
      Detection of human EGFR, phosphorylated at Tyr1068, by immunoblotting. Samples: Whole cell lysate from A431 cells unstimulated (lane 1), or stimulated for EGF (lanes 2-5). Primary antibody: PhosphoDetect™ Anti-Epidermal Growth Factor Receptor (pTyr1068) Rabbit pAb (Cat. No. 324867) (1:1000) pre-incubated without peptide (lanes 1 and 5), with phosphopeptide immunogen (lane 2), a generic phosphotyrosine-containing peptide (lane 3), or non-phosphopeptide corresponding to the immunogen (lane 4). Detection: chemiluminescence.
      References
      ReferencesJo, M., et al. 2000. J. Biol. Chem. 275, 8806.
      Maudsley, S., et al. 2000. J. Biol. Chem. 275, 9572.
      Sieg, D.J. et al. 2000. Nat. Cell. Biol. 2, 249.
      Barbier, A.J. et al. 1999. J. Biol. Chem. 274, 14067.
      Hashimoto, A. et al. 1999. J. Biol. Chem. 274, 20139
      Poppleton, H.M. et al. 1999. Arch. Biochem. Biophys. 363, 227.
      Batzer, A.G. et al. 1994. Mol. Cell. Biol. 14, 5192.
      Product Information
      FormLiquid
      FormulationIn Dulbecco's PBS, 1 mg/ml BSA, 50% glycerol, pH 7.4.
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (1:1000)
      Application CommentsWhen diluted 1:1000 there is sufficient antibody to perform 10 miniblots (10 T) at 10 ml per blot. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Tyr¹⁰⁶⁸ phosphorylation site of human EGFR
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Chicken
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      PhosphoDetect™ Anti-EGFR (pTyr¹⁰⁶⁸) Rabbit pAb FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      PhosphoDetect™ Anti-EGFR (pTyr¹⁰⁶⁸) Rabbit pAb Certificats d'analyse

      TitreNuméro de lot
      324867

      Références bibliographiques

      Aperçu de la référence bibliographique
      Jo, M., et al. 2000. J. Biol. Chem. 275, 8806.
      Maudsley, S., et al. 2000. J. Biol. Chem. 275, 9572.
      Sieg, D.J. et al. 2000. Nat. Cell. Biol. 2, 249.
      Barbier, A.J. et al. 1999. J. Biol. Chem. 274, 14067.
      Hashimoto, A. et al. 1999. J. Biol. Chem. 274, 20139
      Poppleton, H.M. et al. 1999. Arch. Biochem. Biophys. 363, 227.
      Batzer, A.G. et al. 1994. Mol. Cell. Biol. 14, 5192.
      Fiche technique

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision03-March-2008 JSW
      SynonymsAnti-Epidermal Growth Factor Receptor (pTyr¹⁰⁶⁸)
      ApplicationImmunoblotting (1:1000)
      Application Data
      Detection of human EGFR, phosphorylated at Tyr1068, by immunoblotting. Samples: Whole cell lysate from A431 cells unstimulated (lane 1), or stimulated for EGF (lanes 2-5). Primary antibody: PhosphoDetect™ Anti-Epidermal Growth Factor Receptor (pTyr1068) Rabbit pAb (Cat. No. 324867) (1:1000) pre-incubated without peptide (lanes 1 and 5), with phosphopeptide immunogen (lane 2), a generic phosphotyrosine-containing peptide (lane 3), or non-phosphopeptide corresponding to the immunogen (lane 4). Detection: chemiluminescence.
      DescriptionRabbit polyclonal antibody adsorbed against non-phosphorylation site, followed by immunoaffinity chromatography. Recognizes the ~170 kDa EGFR protein phosphorylated at Tyr1068.
      BackgroundEpidermal Growth Factor (EGF) is responsible for a variety of biological effects including mitogenesis and differentiation. The EGF receptor (EGFR) has three domains: the extracellular, transmembrane, and cytoplasmic domains. Upon binding of EGF to the extracellular domain, dimerization of the receptor occurs and the cytoplasmic domains display tyrosine kinnase activity. This leads to autophosphorylation of the ~170 kDa glycoprotein on various residues within the cytoplasmic domain as well as phosphorylation of intracellular substrates. Tyr1068 is an autophosphorylation site in EGFR. The SH2 domain of Grb2 binds to Tyr1068 of EGFR in the multimeric complex EGFR-Grb2-mSos1 that is formed upon stimulation of EGFR with EGF.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Tyr¹⁰⁶⁸ phosphorylation site of human EGFR
      IsotypeIgG
      Specieschicken, human, mouse, rat
      FormLiquid
      FormulationIn Dulbecco's PBS, 1 mg/ml BSA, 50% glycerol, pH 7.4.
      Preservative≤0.1% sodium azide
      CommentsWhen diluted 1:1000 there is sufficient antibody to perform 10 miniblots (10 T) at 10 ml per blot. Variables associated with assay conditions will dictate the proper working dilution.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesJo, M., et al. 2000. J. Biol. Chem. 275, 8806.
      Maudsley, S., et al. 2000. J. Biol. Chem. 275, 9572.
      Sieg, D.J. et al. 2000. Nat. Cell. Biol. 2, 249.
      Barbier, A.J. et al. 1999. J. Biol. Chem. 274, 14067.
      Hashimoto, A. et al. 1999. J. Biol. Chem. 274, 20139
      Poppleton, H.M. et al. 1999. Arch. Biochem. Biophys. 363, 227.
      Batzer, A.G. et al. 1994. Mol. Cell. Biol. 14, 5192.