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ABE1354-100UG Anti-Exo1

Anti-Exo1, Cat. No. ABE1354, is a highly specific rabbit polyclonal antibody that targets Exonuclease 1 and has been tested for use in Immunoprecipitation and Western Blotting.

Anti-Exo1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: EXO1, EXOI, HEX1 UniProt Number: Q9UQ84
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ABE1354-100UG
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIP, WBRbPurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE1354-100UG
      DescriptionAnti-Exo1
      Alternate Names
      • Exonuclease 1
      • hExo1
      • Exonuclease I
      • hExoI
      Background InformationExonuclease 1 (UniProt: Q9UQ84; also known as hExo1, Exonuclease I, hExoI) is encoded by the EXO1 (also known as EXOI, HEX1) gene (Gene ID: 9156) in human. Exonuclease 1 is a magnesium-dependent protein with 5' to 3' exonuclease and an RNase H activity. It may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. It is highly expressed in bone marrow, testis and thymus. Higher levels of expression are also observed in fetal liver. Lower expression has been observed in colon, lymph nodes, ovary, placenta, prostate, small intestine, spleen, and stomach. It functions in DNA mismatch repair to excise mismatch-containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. It interacts physically with the DNA mismatch repair proteins, Msh2 and Mlh1, and is involved in the excision of the mispaired nucleotide. Exonuclease 1 is phosphorylated upon DNA damage and in response to agents stalling DNA replication, probably by ATM or ATR. It is shown to be phosphorylated at Serine 454, Threonine 621, and Serine 714 upon DNA-damage caused by treatment with hydroxyurea, but not upon IR treatment. The hydroxyurea-induced triple phosphorylation of exonuclease 1 facilitates destabilization/degradation of the protein.
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Exo1, Cat. No. ABE1354, is a highly specific rabbit polyclonal antibody that targets Exonuclease 1 and has been tested for use in Immunoprecipitation and Western Blotting.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation Analysis: A representative lot detected Exo1 in HeLa cell lysate (Courtesy of Dr. Zhongsheng You at Washington University in St. Louis).

      Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Exo1 in HeLa and siExo1 hela cell lysates (Courtesy of Dr. Zhongsheng You at Washington University in St. Louis).
      Biological Information
      ImmunogenKLH-conjugted linear peptide corresponding to 16 amino acids from the C-terminal half of human Exonuclease 1. The immunogen sequence is conserved in both isoforms 1 and 2.
      Epitopeunknown
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects Exonuclease 1 in human cells. It targets an epitope within 16 amino acids from the C-terminal half.
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • EXO1
      • EXOI
      • HEX1
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~145 kDa observed; 94.10 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in 293T cells transfected with GFP-Exo1.

      Western Blotting Analysis: 2 µg/mL of this antibody detected Exo1 in 293T cells transfected with GFP-Exo1.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABE1354-100UG 04054839441103