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115934 Eosin B (bluish) (C.I.45400)

115934
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Overview

Replacement Information

Key Spec Table

CAS #EC NumberHill FormulaMolar Mass
548-24-3208-943-1C₂₀H₆Br₂N₂Na₂O₉624.08 g/mol
Description
Catalogue Number115934
SynonymsDibromodinitrofluorescein sodium
DescriptionEosin B (bluish) (C.I.45400)
OverviewEosin B (C.I. 45400) for microscopy Certistain®, is a dry dye for microscopy that is often used as a counterstain, vizualising proteins, connective tissues, fibers and keratin in sample material of human origin. Depending on user requirements and personal preferences, it can be used for the preparation of the commonly used aqueous or alcoholic Eosin B staining solutions. This staining solution will mostly be used for routine staining in the hematoxylin-eosin staining process (H&E). The H&E staining process is the most frequently used staining method for histological material. Due to the hematoxylin solution, the nuclei will appear dark blue to dark violet in color and the Eosin Y solution will stain the cytoplasma and intercellular substances pink to red. As the dye belongs to the portfolio of Certistain® dyes, it is chemically analyzed according to strict specifications before release (CE marked and IVD product) and a high staining quality, with reliable staining results is guaranteed. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
References
Product Information
CAS number548-24-3
EC number208-943-1
Hill FormulaC₂₀H₆Br₂N₂Na₂O₉
Molar Mass624.08 g/mol
HS Code3212 90 00
Structure formula ImageStructure formula Image
Quality LevelMQ400
Applications
Applicationfor microscopy Certistain®
Biological Information
Physicochemical Information
pH value6.2 (10 g/l, H₂O, 25 °C)
Bulk density680 kg/m3
Solubility300 g/l
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Storage class10 - 13 Other liquids and solids
WGKWGK 3 highly hazardous to water
Disposal3
Relatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C.
Safety Information
Product Usage Statements
Storage and Shipping Information
StorageStore at +2°C to +30°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Dye content (gravimetrically)≥ 85 %
Identity
- sodium

passes test
- UV/VIS-Spectrumpasses test
Absorption maximum λ max (water)517 - 525 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.005 g/l; water)800 - 1000
TLC-Testpasses test
Loss on drying (110 °C)≤ 8 %
Suitability for microscopypasses test

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Accessories (Primary)

Catalogue Number Description  
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Categories

IVD/OEM Materials & Reagents > Microscopy > Dyes for Microscopy > Certistain® Dyes
Reagents, Chemicals and Labware > Inorganic Reagents > Safety Products and General Applications > Indicators > Acid Alkali Indicators or pH Indicators