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115943 Methylene blue (C.I.52015)

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115943
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Overview

Replacement Information

Key Spec Table

CAS #EC NumberHill FormulaChemical FormulaMolar Mass
61-73-4200-515-2C₁₆H₁₈ClN₃S * x H₂OC₁₆H₁₈ClN₃S * x H₂O (x=2-3)319.86 g/mol (anhydrous)
Description
Catalogue Number115943
SynonymsMethylthioninium chloride, 3,7-Bis(dimethylamino)phenothiazinium chloride, Tetramethylthionine chloride
DescriptionMethylene blue (C.I.52015)
OverviewMethylene blue (C.I. 52015) for microscopy Certistain® is a dry dye that is used in bacteriology for an overview stain and as counterstaining for Ziehl-Neelsen method in sample material of human origin. Bacteria appear blue. The dye has a further important application in hematology: Methylene blue and eosin are the main components of many staining solutions and dye mixtures. Certistain® is the product group for seleceted high quality dyes that fulfill all quality requirements of IVD and CE registration. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
References
Product Information
CAS number61-73-4
EC number200-515-2
Hill FormulaC₁₆H₁₈ClN₃S * x H₂O
Chemical formulaC₁₆H₁₈ClN₃S * x H₂O (x=2-3)
Molar Mass319.86 g/mol (anhydrous)
HS Code3212 90 00
Structure formula ImageStructure formula Image
Quality LevelMQ400
Applications
Applicationfor microscopy Certistain®
Biological Information
Physicochemical Information
Melting Point180 °C (decomposition)
pH value3 (10 g/l, H₂O, 20 °C)
Bulk density400 - 600 kg/m3
Solubility50 g/l
Dimensions
Materials Information
Toxicological Information
LD 50 oralLD50 Rat 1180 mg/kg
Safety Information according to GHS
Hazard Pictogram(s) Hazard Pictogram(s)
Hazard Statement(s)H302: Harmful if swallowed.
Precautionary Statement(s)P264: Wash skin thoroughly after handling.
P270: Do not eat, drink or smoke when using this product.
P301 + P312: IF SWALLOWED: Call a POISON CENTER/doctor if you feel unwell.
P501: Dispose of contents/ container to an approved waste disposal plant.
Signal WordWarning
Storage class10 - 13 Other liquids and solids
WGKWGK 2 obviously hazardous to water
Disposal3
Relatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C.
Safety Information
Categories of dangerharmful
Product Usage Statements
Storage and Shipping Information
StorageStore at +2°C to +30°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Dye content (spectrophotometrically)≥ 82 %
Identity
- Chloride

passes test
- UV/VIS-Spectrumpasses test
Absorption maximum λ max (ethanol 50 %)660 - 665 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.003 g/l; ethanol 50 %)2250 - 2750
TLC-Testpasses test
Loss on drying (110 °C)10 - 15 %
Suitability for microscopypasses test

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Categories

IVD/OEM Materials & Reagents > Microscopy > Dyes for Microscopy > Certistain® Dyes
Reagents, Chemicals and Labware > Inorganic Reagents > Safety Products and General Applications > Indicators > Redox Indicators