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EZHIL8

Human IL-8 ELISA Kit

Name: Human IL-8 ELISA Kit
Brand: MM

detect IL-8 in 50 μL serum, plasma or cell culture supernatent

別名:

Granulocyte chemotactic protein 1, IL-8, Interleukin-8, Monocyte-derived neutrophil-activating peptide, Neutrophil-activating protein 1, T-cell chemotactic factor

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この商品について

UNSPSC Code:

41116158

NACRES:

NA.84

Gene information:

human ... CXCL8(3576)

Input:

sample type plasma (K2 EDTA)
sample type cell culture supernatant
sample type serum

Species reactivity:

human

Storage temp.:

2-8°C

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特性

製品名

Human IL-8 Conferma^® ELISA, quantification of Human IL-8 in serum, plasma, or culture media samples

Quality Level

200

species reactivity

human

parameter

50 μL sample volume (4hr assay)

technique(s)

ELISA: suitable

input

sample type plasma (K2 EDTA)
sample type cell culture supernatant
sample type serum

UniProt accession no.

P10145

application(s)

research use

assay range

accuracy: 77-102 (plasma)%
linearity: 105 (plasma)%, inter-assay cv: <4.7%
intra-assay cv: <4.0%
standard curve range: 0.82-200 pg/mL
accuracy: 70-105 (serum)%
linearity: 94-106 (serum)%

detection method

colorimetric (450nm/590nm)

storage temp.

2-8°C

Gene Information

human ... CXCL8(3576)

詳細

General description

IL-8 is secreted by mononuclear macrophages, neutrophils, eosinophils, T lymphocytes, epithelial cells, and fibroblasts. It functions as a chemotactic factor by guiding the neutrophils to the site of infection. IL-8 also participates with other cytokines in the proinflammatory signaling cascade. The protein is implicated in numerous pathologies including, bacterial, cancer, cardiovascular and viral diseases.
IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.

This is a 96 well format sandwich ELISA which uses our “Conferma” process to ensure a high degree of lot to lot consistency. The process involves the physiochemical characterization of the ELISAs critical reagents lot to lot, with finished kits being evaluated using a sample library to bridge between lots.

Application

This Human Interleukin-8 (IL-8) ELISA kit is used for the quantification of Human IL-8 in serum, plasma, or culture media samples.

Analytes available:

IL-8

Research Category:

Cytokines
Inflammation & Immunology
Immune Response
Metabolic Disease

Analysis Note

3 hour 45 minute assay, room temperature, 50μl of neat serum or plasma is recommened.

Legal Information

CONFERMA is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

For research use only. Not for use in diagnostic procedures.

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プロトコル

ELISAプロトコル

このページでは、以下の4つの一般的なELISA法を説明します。サンドイッチELISAの手順、リン酸化アッセイの手順、EIAアッセイの手順、細胞ベースアッセイの手順

ELISA Protocols

This article offers 4 popular ELISA protocols: Sandwich ELISA protocol, Phosphorylation Assay Procedure, EIA Assay Procedure, & Cell-based Assay Procedure.

資料

Conferma^® ELISAs: Designed for Sample Detection & Lot Consistency

Learn about the Conferma^® ELISA development and manufacturing methods that provide strong sample detection and long-term assay and lot consistency, giving you confidence in your research.

グローバルトレードアイテム番号

カタログ番号	GTIN
EZHIL8-100K	04065265645669
EZHIL8-100K10PK	04065265645683
EZHIL8-100K5PK	04065265645676