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EZMCP1

Human MCP-1 ELISA Kit

Name: Human MCP-1 ELISA Kit
Brand: MM

detects MCP-1 in 50 μL serum, plasma or cell culture supernatent

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この商品について

UNSPSC Code:

41116158

NACRES:

NA.84

Gene information:

human ... CCL2(6347)

Input:

sample type plasma (K2 EDTA)
sample type serum
sample type cell culture supernatant

Species reactivity:

human

Storage temp.:

2-8°C

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特性

製品名

Human MCP-1 Conferma^® ELISA, quantification of human MCP-1 in serum, plasma, or culture media samples.

clone

monoclonal

Quality Level

200

species reactivity

human

parameter

50 μL sample volume (4hr assay)

technique(s)

ELISA: suitable

input

sample type plasma (K2 EDTA)
sample type serum
sample type cell culture supernatant

UniProt accession no.

P13500

application(s)

research use

assay range

linearity: 94-106 (serum)%, linearity: 94-110 (plasma)%, inter-assay cv: <12.9%
intra-assay cv: <4.9%
standard curve range: 1.17-300 pg/mL
accuracy: 84-95%

detection method

colorimetric (450nm/590nm)

storage temp.

2-8°C

Gene Information

human ... CCL2(6347)

詳細

General description

Human MCP-1 is a chemokine assoscited with the attraction of Monocytes and Basophils to the site of a pathology. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. In particilar this is a marker of inflammatory disorders. It binds to chemokine receptors CCR2 and CCR4.
This is a 96 well format sandwich ELISA which uses our Conferma^® process to ensure a high degree of lot to lot consistency. The process involves the physiochemical characterization of the ELISAs critical reagents lot to lot, with finished kits being evaluated using a sample library to bridge between lots.

Application

This Human MCP-1 Conferma^® ELISA kit is used for the quantification of Human MCP-1 in serum, plasma, or culture media samples. One kit is sufficient to measure 37 unknown samples in duplicate.

Research Category:

Cytokines
Inflammation & Immunology
Immune Response
Metabolic Disease

Packaging

Two boxes; each to be analyzed separately.

Analysis Note

3 hour 45 minute assay, room temperature, 1:5 dilution recommended e.g.30 μL sample + 120μL of diluent (serum, plasma, or tissue culture medium)

Legal Information

CONFERMA is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

For research use only. Not for use in diagnostic procedures.

pictograms

GHS05,GHS07,GHS08

signalword

Danger

hcodes

H290 - H302 + H312 + H332 - H317 - H318 - H373 - H412

pcodes

P273 - P280 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338 - P314

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Met. Corr. 1 - Skin Sens. 1 - STOT RE 2

target_organs

Respiratory Tract

保管分類

8A - Combustible, corrosive hazardous materials

wgk

WGK 3

試験成績書（COA）

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プロトコル

ELISAプロトコル

このページでは、以下の4つの一般的なELISA法を説明します。サンドイッチELISAの手順、リン酸化アッセイの手順、EIAアッセイの手順、細胞ベースアッセイの手順

ELISA Protocols

This article offers 4 popular ELISA protocols: Sandwich ELISA protocol, Phosphorylation Assay Procedure, EIA Assay Procedure, & Cell-based Assay Procedure.

資料

Conferma^® ELISAs: Designed for Sample Detection & Lot Consistency

Learn about the Conferma^® ELISA development and manufacturing methods that provide strong sample detection and long-term assay and lot consistency, giving you confidence in your research.

グローバルトレードアイテム番号

カタログ番号	GTIN
EZMCP1-99K5PKRM	04061841718567
EZMCP1-99K10PKRM	04061841718574
EZMCP1-99KRM	04061841718550