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Merck

85927

ECC ChromoSelect Selective Agar

NutriSelect® Plus, suitable for microbiology

別名:

E. coli-Coliform Selective Agar ChromoSelect, ECC Selective Agar ChromoSelect

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この商品について

UNSPSC Code:
41171606
NACRES:
NA.85
MDL number:
MFCD01634645

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sterility

non-sterile

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 10.0 g/L , casein enzymic hydrolysate, 3.3 g/L , chromogenic mixture, 0.43 g/L , disodium hydrogen phosphate, 1 g/L , peptone, special, 6.0 g/L , sodium chloride, 2 g/L , sodium dihydrogen phosphate, 0.6 g/L , sodium pyruvate, 1 g/L , sorbitol, 1 g/L , Tergitol™
7, 0.15 g/L , tryptophan, 1 g/L

manufacturer/tradename

NutriSelect® Plus

final pH

6.8±0.2 (25 °C)

application(s)

agriculture
bioburden testing
environmental
food and beverages
water monitoring, microbiology

suitability

selective and differential for Citrobacter spp., selective and differential for Enterobacter spp., selective and differential for Escherichia coli, selective and differential for Salmonella spp., selective and differential for Shigella spp., selective and differential for coliforms, selective and differential for enterobacteriaceae

Quality Level

200

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関連するカテゴリー

Application

ECC ChromoSelect Selective Agar is a selective medium recommended for the simultaneous detection of Escherichia coli and coliforms in water and food samples. The ingredients help even the sublethally injured coliforms to grow rapidly, Tergitol™ inhibits Gram-positive as well as some Gram-negative bacteria other than coliforms. The chromogenic mixture contains two chromogenic substrates as Salmon-GAL and X-glucuronide. The enzyme β-D-galactosidase produced by coliforms cleaves Salmon-GAL, resulting in the salmon-to-red coloration of coliform colonies. The enzyme β-D-glucuronidase produced by E. coli, cleaves X-glucuronide. E. coli forms dark blue-to-violet colored colonies due to cleavage of both Salmon-GAL and X-glucuronide. The addition of tryptophan improves the indole reaction. To confirm E. coli, add a drop of Kovac′s reagent (Catalog No. 60983) on the dark blue-to-violet colony. Formation of cherry-red color indicates the positive reaction.

Preparation Note

Suspend 26.5 g in 1 liter of distilled water. Heat in a boiling water bath or in a free flowing steam, with stirring to dissolve the medium completely (approximately 35 minutes). Medium may show haziness, but it does not affect the performance. If desired to inhibit Pseudomonas and Aeromonas species, add 5 mg of cefsulodin for surface and pour plate method and 10 mg of cefsulodin for membrane filter technique. Mix well.
Suspend 26.5 g in 1 litre distilled water. Heat in a boiling water bath or in a free flowing steam, with stirring to dissolve the medium completely (approximately 35 minutes). Medium may show haziness, but it does not affect the performance. If desired to inhibit Pseudomonas and Aeromonas species add 5 mg Cefsulodin (Cat. No. 22126) for surface and pour plate method and 10 mg Cefsulodin for membrane filter technique. Mix well.

Other Notes

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
TERGITOL is a trademark of The Dow Chemical Company ("Dow") or an affiliated company of Dow

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317

Hazard Classifications

Skin Sens. 1

保管分類

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

85927-26.5G-F: + 85927-VAR-F: + 85927-BULK-F: + 85927-500G-F: + 85927-VAR:

jan

最新バージョンのいずれかを選択してください：

試験成績書（COA）

Lot/Batch Number
BCCN2022
BCCL3003
BCCH4374
BCCG1479
BCCB8207

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文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Hongda Wang et al.
Light, science & applications, 9, 118-118 (2020-07-21)
Early identification of pathogenic bacteria in food, water, and bodily fluids is very important and yet challenging, owing to sample complexities and large sample volumes that need to be rapidly screened. Existing screening methods based on plate counting or molecular

資料

Differentiation and Detection of Escherichia coli, Coliforms and Enterobacteriaceae

An article concerning selective growth media for differentiation and detection of Escherichia coli and other coliforms.

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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