T7659
トリプシンインヒビター(1×)溶液, 調製品
Animal component free, BioReagent, suitable for cell culture
製品名
トリプシンインヒビター(1×)溶液, 調製品, Animal component free, BioReagent, suitable for cell culture
sterility
sterile-filtered
product line
BioReagent
form
solution
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−20°C
Quality Level
Application
ケラチノサイトの継代培養に使用できる本製品は、クニッツ型大豆トリプシンインヒビター(SBTI)を含有し、トリプシンやトリプターゼなどのセリンプロテアーゼの触媒活性を阻害します。細胞培養に使用して、継代中基層からの細胞剥離に用いられるトリプシンの作用を停止させます。その他、抗セリンプロテアーゼの研究にも有用であると考えられます。
トリプシン／EDTA溶液の作用を中和するために使用されます。
Physical form
98.7% DPBSおよび1.3% 大豆トリプシンインヒビタ-溶液
Preparation Note
TID:トリプシン-EDTAの最小の量比は1:1としてください。
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
保管分類
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Sarra Djemil et al.
Cellular and molecular neurobiology, 41(8), 1787-1799 (2020-08-30)
Septal innervation of basal forebrain cholinergic neurons to the hippocampus is critical for normal learning and memory and is severely degenerated in Alzheimer's disease. To understand the molecular events underlying physiological cholinergic synaptogenesis and remodeling, as well as pathological loss
I B Svendsen et al.
Carlsberg research communications, 54(6), 231-239 (1989-01-01)
A trypsin inhibitor with a Km of 5 x 10(-5) M has been isolated from kohlrabi (Brassica napus var. rapifera). Subtilisin DY is inhibited only weakly and chymotrypsin not at all. The inhibitor is closely related to napin as determined
C L Dumke et al.
Journal of applied physiology (Bethesda, Md. : 1985), 92(2), 657-664 (2002-01-18)
Serum proteins [molecular weight (MW) > 10,000] are essential for increased insulin-stimulated glucose transport after in vitro muscle contractions. We investigated the role of the kallikrein-kininogen system, including bradykinin, which is derived from kallikrein (MW > 10,000)-catalyzed degradation of serum
Maurizio Trovato et al.
Biochemical and biophysical research communications, 302(2), 311-315 (2003-02-27)
The design of minimal units required for enzyme inhibition is a major field of interest in structural biology and biotechnology. The successful design of the cyclic dodecapeptide corresponding to the Phe17-Val28 reactive site amino acid sequence of the low-molecular-mass trypsin
プロトコル
Natural trypsin inhibitors (serpins) regulate protein activation and catabolism by inhibiting serine proteases in vivo.
天然のトリプシンインヒビター（セルピン）は、in vivoでタンパク質の活性および異化をコントロールします。
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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