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In contrast to conventional particle-packed columns, Merck’s Chromolith® HPLC columns are based on state-of-the-art monolithic silica technology. Produced from a continuous piece of porous silica using a sol-gel process, Chromolith® rod columns possess a defined bimodal pore structure with macro- and mesopores in the micro- and nanometer range. The high permeability and porosity of the silica skeleton and the resulting low back pressure allow for more flexible flow rates compared to particulate columns. As a result, Chromolith® HPLC columns enable high-throughput analysis without loss of separation efficiency or peak capacity.

Merck:/Freestyle/LE-Lab-Essentials/Chromatography/LE-Chromolith HPLC Columns-200x135-08282013.jpgThe revolutionary bimodal pore structure of Chromolith® HPLC columns provides a unique combination of macropores and mesopores.

The mesopores form the fine porous structure (average pore size 13 nm) and create the large uniform surface area on which adsorption takes place, thereby enabling high-performance chromatographic separation. The macropores (average size 2 μm) allow rapid flow of the mobile phase at low pressure.

Features and Benefits

1. Speed of analysis
  • Separations two times faster at half the column back pressure with Chromolith® compared to 5 μm columns
  • Higher sample throughput – separations up to nine times faster if required
  • Fast column re-equilibration between analyses
2. Flow flexibility and flow gradients
  • Quick response to the flow change
  • Wide optimal flow ranges
3. Improved HPLC system security: robustness, reliability and versatility
  • Significantly increased column lifetime
  • Reduced maintenance on HPLC pump and injector seals
  • Reduced need for sample preparation as columns are very resistant to blocking, even with biological samples
4. Column length no longer pressure limited
  • Very high separation efficiency by column coupling
  • Variety of column lengths available for samples of different complexities
5. Cost savings due to increased sample throughput 
  • Justifies the expense of a method revalidation within one month
6. Chromolith® HPLC columns are ideally suited for use with all standard HPLC instruments
  • Cladded Chromolith® columns in PEEK are very easy to use and to handle

Classic Particulate Columns Versus Chromolith® HPLC Columns

The use of HPLC columns containing the classic 3 or 5 μm small silica particles often results in high back pressure. This may damage both the column and the HPLC system; therefore, classic HPLC columns have limited length and a limited number of theoretical plates. Attempts have been made to increase the plate count by decreasing the particle size, but this results in unacceptable back pressure and limits the variety of separations that can be satisfactorily achieved.

The development of faster separation processes has become one of the most important issues in high-performance liquid chromatography. Modern laboratories utilize automation of HPLC systems which feature around the clock operations . To accelerate the process even more, there’s no better choice than Chromolith® HPLC columns. Thanks to their revolutionary bimodal pore structure, Chromolith® columns provide excellent separations in a fraction of the time that a standard particulate column takes. This enables higher speeds and higher sample throughput at lower back pressure.

Scientific Papers on Working with Chromolith®

Merck:/Freestyle/LE-Lab-Essentials/Chromatography/LE-Scientific Papers-150x112-12112013.jpgScientist in laboratories around the world have published articles after solving difficult analysis problems using Chromolith® HPLC columns. Perhaps their experience can help you to further your own research by providing examples of specific separation conditions.

1. Comparison of the performance of Chromolith® Performance RP-18e, 1.8-mm
Zorbax Eclipse XDB-C18 and XTerra MSC18, based on modelling approaches
Pous-Torres, S.; Torres-Lapasio, J. R.; Garcia-Alvarez-Coque, M. C.
Analytical and Bioanalytical Chemistry, 405(7), 2013, 2219-2231

2. Determination of Preservatives in Cosmetics, Cleaning Agents and Pharmaceuticals Using Fast Liquid Chromatography
Baranowska Irena; Wojciechowska Iwona; Solarz Natalia; Krutysza Ewa
Journal of chromatographic science, 2013 

3. High-resolution monolithic columns-a new tool for effective and quick separation
Sklenarova, Hana; Chocholous, Petr; Koblova, Petra; Zahalka, Lukas; Satinsky, Dalibor; Matysova, Ludmila; Solich, Petr
Analytical and Bioanalytical Chemistry, 405(7), 2013, 2255-2263 

4. LC-MS/MS-ESI method for simultaneous quantitation of metformin and repaglinidie in rat plasma and ist application to pharmacokinetic study in rats
Sharma, Kuldeep; Pawar, Gopal; Yadam, Swetha; Giri, Sanjeev; Rajagopal, Sriram; Mullangi, Ramesh
Biomedical Chromatography, 27(3), 2013, 356-364 

5. A novel LC-MS/MS method for simultaneous quantification of tenofovir and lamivudine in human plasma and its application to a pharmacokinetic study
Matta, Murali Krishna; Burugula, Laxminarayana; Pilli, Nageswara Rao; Inamadugu, Jaswanth Kumar; Seshagiri Rao, J. V. L. N
Biomedical Chromatography, 26(10), 2012, 1202-1209
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