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A Technical Resource and Product Guide – 
All in One Convenient Book
Essential Biochemicals for Research. Resource for scientists for the preparation and use of research essential biochemicals.Now everything you need to know about the preparation and use of research essential biochemicals, such as antibiotics, buffers, detergents, dyes, stains and substrates, are in one convenient book.

Download the PDF for your lab!

Buffers, Dyes & Probes

Almost all biological processes are pH-dependent. Even a slight change in pH can result in metabolic acidosis or alkalosis, resulting in severe complications. The purpose of a buffer in a biological system is to maintain intracellular and extracellular pH within a very narrow range and resist changes in pH in the presence of internal and external influences. Read the Background information to learn more about buffers and how they control hydrogen ion concentrations.

Dyes, Stains, and Probes
Cell visualization and microscopy are fundamental tools for investigating cellular behavior and structure, including changes in structural features, as well as cellular and sub-cellular dynamics under normal and pathological conditions. As the technology for microscopy has evolved, the ability to interrogate discrete protein and organelle dynamics has increased and the need for both general and specific visualization tools has grown. Dyes and stains provide rich information on cellular and subcellular processes, especially when used in conjunction with fluorescently-conjugated primary and secondary antibodies.

Advantages of Calbiochem® Buffers, Dyes, Stains, and Probes:

  • Are available in various grades and package sizes for greater flexibility
  • Offer highly purified substances, to ensure reproducible results from the lab bench to production
  • Are trusted, as demonstrated through thousands of citations
  • Are manufactured in a highly controlled environment, ensuring the utmost purity and quality
Browse all Buffers
We are continuing to expand our line of OmniPur® and ULTROL® grade buffer materials, which are of superior quality and are manufactured to meet our stringent specifications. ULTROL® grade buffers, whenever possible, are screened for uniform particle size, giving uniform solubility characteristics. OmniPur® grade reagents are DNase, RNase, and protease tested to ensure the integrity of your starting material and to improve downstream results.

Browse all dyes, stains and probes products

Background – Resource and information for the preparation and use of buffers
  • Criteria for Suitable Biological Buffers
  • Preparation of Some Common Buffers for Use in Biological Systems
  • Some Useful Tips for pH Measurements
Quick Reference Guides – Useful tables, figures, and conversion charts