Protein Quantitation with Direct Detect® Spectrometer |
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Quantitation Approach | Assay Range | Sample Volume (tube/plate) | Amount Protein Required to Measure 1 mg/mL | Compatible |
---|---|---|---|---|
Direct Detect® Spectrometer | 250-5,000 μg / mL | 2 μL | 6 μg | Detergents Reducing agents SDS Chelators |
Bradford Assay | 100-1,500 μg / mL | 35 μL / 7 μL | 21 μg | Most reducing agents Chelators |
BCA Assay | 20-2,000 µg / mL | 50 µL / 25 µL | 30 μg | Detergents |
Micro BCA™ Assay | 2-40 µg / mL | 500 µL / 150 µL | 450 μg | Detergents |
Lowry Assay | 10-1,500 μg / mL | 200 μL / 40 μL | 120 μg | SDS |