Western Blotting Application Notes |
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Antibody Reuse: Antibody Recovery and Reuse with the SNAP i.d.® Protein Detection SystemRecovering the primary antibody for possible reuse can be very valuable for Western blotting experiments. Here, we show that greater than 90% of the primary antibody can be recovered after the incubation step by following the recommended protocol. The antibodies collected in the SNAP i.d.® 2.0 System can be used successfully in subsequent immunodetection with no reduction in blot quality, even after extended incubations.
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Immunodetection: Key Steps for Successful Immunodetection Using the SNAP i.d.® Protein Detection SystemThe Western blot is a powerful tool used extensively in protein research to detect and compare the relative levels of proteins without the need for their prior purification. Its widespread appeal is based on its overall simplicity, coupled with the high resolution of proteins separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) prior to membrane transfer.
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Fluorescent Immunodetection: Fluorescent Immunodetection Using the SNAP i.d.® Protein Detection SystemRecent advances in fluorescent dye chemistry and blotting membranes coupled with improvements in instrumentation have accelerated the application of fluorescence detection methodologies to Western blotting. The SNAP i.d.® Protein Detection System has been developed to substantially shorten the time required for fluorescent immunodetection through the use of vacuum. |