Chromolith® RP-8 Endcapped Monolithic
Silica HPLC Columns
Chromolith® RP-8 Endcapped HPLC Columns |
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Superior Retention for Hydrophobic Compounds
Chromolith® RP-8 endcapped columns provide better retention for hydrophobic compounds than RP-18 endcapped columns. Due to their shorter alkyl chains, they offer less retention and slightly different selectivity. This makes it possible to achieve baseline separations
on RP-8 endcapped bonded columns. In comparison, no separation at all is observed on RP-18 endcapped bonded silica columns under identical elution conditions.
Otherwise, Chromolith® RP-8 endcapped columns offer the same outstanding benefits as Chromolith® RP-18 endcapped: high throughput, extremely long column lifetime, lower matrix sensitivity and added column performance through column coupling. Based on high-purity silica, the columns minimize the negative effects of trace metals. Furthermore, they are chemically modified with n-alkyl chains, which possess a high ligand density, and fully endcapped in order to reduce the effects of unmodified silanol groups.
Features and Benefits
- Better retention for hydrophobic compounds
- High throughput at high flow rates with the best overall column quality
- Possibility of flow gradients
- Added column performance by column coupling
- Rigid monolithic structure for a longer column lifetime
- Lower matrix sensitivity
Specifications
| Silica type | High-purity |
|---|---|
| Particle size | Monolithic |
| Macropore size | 2 μm |
| Mesopore size | 13 nm (130 Å) |
| Pore volume | 1.0 mL/g |
| Total porosity | > 80% |
| Surface area | 300 m2/g |
| Surface modification | RP-8 endcapped |
| Carbon content | 11% |
Applicatgion Example Chromolith® Performance RP-8e
| Column | Chromolith® Performance RP-8 endcapped, 100-4.6 mm | ||
|---|---|---|---|
| Mobile phase | A: Acetonitrile/ water 90/ 10 + 0.1% TFA B: 0.1% TFA in water |
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| Gradient | Time/min | %A | %B |
| 0.0 | 45 | 55 | |
| 1.0 | 90 | 10 | |
| 3.0 | 90 | 10 | |
| Flow rate | 2 mL/min | ||
| Pressure | 30 -40 bar | ||
| Detection | 214 nm | ||
| Temperature | Ambient | ||
| Injection volume | 30 μl | ||
| Sample |
1. (Sar1, Ala8)-Angiotensin II |
87 μg/mL |
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| Column | Chromolith® Performance RP-8 endcapped, 100-4.6 mm | ||
|---|---|---|---|
| Mobile phase | A: Acetonitrile B: 20mM NaH2PO4 pH 2.5 |
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| Gradient | Time/min | %A | |
| 0.0 | 2 | ||
| 0.5 | 18 | ||
| 8.5 | 18 | ||
| 9.1 | 32 | ||
| 16 | 32 | ||
| Flow rate | 1 mL/min | ||
| Pressure | 23 bar | ||
| Detection | 220 nm | ||
| Temperature | Ambient | ||
| Injection volume | 5 μl | ||
| Sample |
1. Malic acid |
0.92 mg/mL |
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