Chromolith® SemiPrep HPLC Columns

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Perfect Scale-Up from Analytical to Preparative LC

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Chromolith® SemiPrep HPLC columns are ideally suited for direct scale-up from analytical to semi-prep. They offer faster sample throughput at a lower operating pressure compared to semi-prep columns packed with 5 μm particles. The columns are also excellent for solving separation problems with non-polar basic and acidic compounds, as well as peptides.

With an internal diameter (i.d.) of 10 mm, Chromolith® SemiPrep columns are the perfect alternative to particulate columns of 10 mm i.d. (and even 21.2 mm i.d). Furthermore, the selectivity of Chromolith® RP-18 endcapped SemiPrep columns is comparable to common RP-18 endcapped reversed phase columns.

Bimodal Porous Silica Rod Structure

The columns also offer an extended lifetime thanks to their rugged monolithic silica structure, which is the same as that of Chromolith® analytical columns with an internal diameter of 4.6 mm. The 2 μm macropores of the silica rod dramatically reduce separation time, while the 13 nm mesopores create a large surface area for adsorption of target molecules.

Specifications

Features and Benefits

  • Direct scale-up from analytical to semi-prep
  • Faster sample throughput at a lower operating pressure compared to semi-prep columns packed with 5 μm particles
  • Sharp separations, even at high sample loading
  • Excellent column lifetime thanks to the rugged monolithic silica structure
  • Optimized for LC/MS by a surface modification process minimizing column bleeding

Optimum Separation at Various Flow Rates

Chromolith® SemiPrep RP-18 endcapped 100-10 mm i.d. columns combine high separation speed with excellent performance. They are the ideal alternative to particulate columns with 10 and 21.2 mm i.d.

Separation of a standard mixture

Sample Loadability

Chromolith® SemiPrep HPLC columns deliver sharp separations, even at high sample loading.

Separation of 80 mg/injection

Up-Scaling

A Chromolith® SemiPrep RP-18 endcapped column injected with a 25 mg sample demonstrates the same excellent separation properties as a corresponding analytical column.

Up-Scaling

 
 
 
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