コンテンツへスキップ
Merck

D7440

JumpStart™Taq ReadyMix™ 定量PCR用

For probe-based real-time PCR

別名:

hot start DNA polymerase, hot start PCR, hot start master mix, qPCR, real time quantitative PCR

ログインで組織・契約価格をご覧ください。

サイズを選択してください

この商品について

NACRES:
NA.55
UNSPSC Code:
41106300

主要文書

すべての文書を表示
テクニカルサービス
お困りのことがあれば、経験豊富なテクニカルサービスチームがお客様をサポートします。
お手伝いします
テクニカルサービス
お困りのことがあれば、経験豊富なテクニカルサービスチームがお客様をサポートします。
お手伝いします

製品名

JumpStart™Taq ReadyMix™ 定量PCR用, For probe-based real-time PCR

form

liquid

usage

sufficient for 100 reactions
 sufficient for 20 reactions
 sufficient for 400 reactions

feature

Multiplex PCR
dNTPs included
hotstart

concentration

2.5 units/reaction (50 μL reaction volume)

technique(s)

qPCR: suitable

color

colorless

input

purified DNA

application(s)

agriculture

compatibility

Bio-Rad MyiQ ( )
 Bio-Rad iCycler iQ
 Bio-Rad iQ 5
for use with ABI 5700
for use with ABI 7000
for use with ABI 7300
for use with ABI 7500 Fast
for use with ABI 7500
for use with ABI 7700
for use with ABI 7900 Fast
for use with ABI 7900 HT
for use with ABI 7900
for use with ABI StepOne
for use with ABI StepOnePlus
for use with ABI ViiA 7
for use with Bio-Rad CFX384
for use with Bio-Rad CFX96
for use with Bio-Rad MJ Chromo4
for use with Bio-Rad MJ Opticon 2
for use with Bio-Rad MJ Opticon Cepheid SmartCycler
for use with Bio-Rad MJ Opticon
for use with Bio-Rad MiniOpticon
for use with Eppendorf® Mastercycler ep realplex2 s
for use with Eppendorf® Mastercycler ep realplex
for use with Illumina Eco qPCR
for use with Qiagen Corbett Rotor-Gene 3000
for use with Qiagen Corbett Rotor-Gene 6000
for use with Qiagen Corbett Rotor-Gene Q
for use with Roche LightCycler 480
for use with Strategene Mx3000P
for use with Strategene Mx3005P
for use with Strategene Mx4000

detection method

probe-based

shipped in

wet ice

storage temp.

−20°C

Quality Level

200

関連するカテゴリー

Legal Information

(i)認可された5' Nuclease Core Kitとライセンスを受けたプローブを両方購入した場合、(ii)ライセンスを受けた5' Nuclease Kitを購入した場合、(iii)Applied Biosystemsからライセンス権を購入した場合、特許で保護されている5' Nuclease Processを研究で使用するライセンスが得られます。

本製品は、認可された5' Nuclease Core Kitです。本製品を使用する場合、米国特許番号 5,210,015、5,487,972と、対応する米国外での特許請求のうち、1つ以上が適用されます。本製品の購入について、購入者自身の内部研究のために購入した量を使用する場合に限り、前述の特許請求に基づく訴訟から免責されますが、これは限定的なものであり、譲渡することはできません。ライセンスを受けたプローブを別途購入することによって、米国特許番号5,538,848、5,723,591、5,876,930、6,030,787、6,258,569、5,804,375(請求項1～12のみ)、対応する米国外の特許請求、米国特許番号6,214,979に対応する米国外請求に基づく権利が譲渡されます。他の特許によって保護されている権利や、あらゆる種類の商業サービス(購入者の研究結果を報酬やその他の商業的事由のために無制限に報告することを含む)を行う権利が、暗示や禁反言によって明示的に譲渡されるわけではありません。本製品は研究専用です。Rocheの特許に基づき診断に利用する場合は、別途Rocheからライセンスを受ける必要があります。ライセンス購入に関する詳細な情報については、ライセンス管理者(Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA)にお問い合わせ下さい。
Eppendorf is a registered trademark of Eppendorf AG
JumpStart is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
SYBR is a registered trademark of Thermo Fisher Scientific or its subsidiaries

Other Notes

1 unitは、74°C、30分間に、10 nmolの全dNTPを酸不溶性DNAに取り込む酵素量です。
JumpStart Taq ReadyMix for Quantitative PCR combines the advantages of a hot start enzyme with a ready-to-use mix for high throughput, quantitative PCR (qPCR). It is formulated without a detection chemistry making it suitable for use with a variety of formats including dual-labeled probes, molecular beacons, or double stranded binding dyes such as SYBR Green I.

Application

JumpStart™ Taq ReadyMix™ for Quantitative PCR has been used:

  • for quantitative polymerase chain reaction (qPCR) amplification of HIV-1 RNA that is reverse transcribed and DNA using probes
  • in a 2-step RT-qPCR assay for the quantification of reverse transcribed RNA in a study to monitor the activity of licorice in Treg cell differentiation and function
  • as a component of the reaction mixture for the detection and amplification of Salmonella sp. and internal amplification control (IAC) pUC 19 plasmid DNA
  • as a component of the reaction mixture for the detection of Clostridium difficile by quantitative polymerase chain reaction (qPCR)
  • for quantitative real-time -PCR of reverse-transcribed ß-arrestin-1 (ARRB1) and β-arrestin-2 (ARRB2) mRNA

Biochem/physiol Actions

The ReadyMix contains JumpStart Taq DNA Polymerase, 99% pure deoxynucleotides, and buffer in an optimized 2× concentrate. To prepare a reaction, 25 μL of the ReadyMix is added to primers, template, detection chemistry, and water for a total reaction volume of 50 μL. Set up can be performed at room temperature since the JumpStart Taq antibody renders the Taq DNA polymerase inactive. During the first denaturation cycle, the antibody dissociates from the enzyme and full activity is restored. No special preparations or protocol changes are required.

Features and Benefits

  • Ultimate Convenience: ReadyMixes contain all components necessary for QPCR, simply add fluorescent detection chemistry, primers, and template
  • Greater Specificity & Increased Target Yield: JumpStart™ Taq prevents non-specific amplification and increased target yield
  • Maximum Flexibility: Suitable for use with a variety of detection chemistries including molecular probes and double-stranded binding dyes such as SYBR® Green I
  • Designed for use with either plate/tube real-time thermal cyclers or capillary instruments

General description

JumpStart™ Taq ReadyMix™ is a ready-to-use 2X master mix that contains JumpStart™ Taq DNA polymerase, 99% pure dNTPs, reaction buffer and JumpStart™ Taq antibody. It combines the performance enhancements of our JumpStart™ Taq Antibody for hot start PCR with the convenience of an easy-to-use reaction mixture. Since it has no added dyes, this is the ideal solution for performing high-throughput, quantitative PCR methods that rely on a fluorescent probe. The Taq DNA polymerase is an antibody-inactivated hot-start enzyme. Once the reaction temperature reaches 70°C, the DNA polymerase-antibody complex dissociates and Taq DNA polymerase activity is restored. This antibody-enzyme complex allows for easy and convenient set-up with less contamination risk than manual hot-start techniques.

Packaging

Sigma′s Reference Dye for Quantitative PCR is included separately with this ReadyMix for normalization of the reaction data. The dye has a maximum excitation of 586 nm, and a maximum emission of 605 nm. The instrument settings for ROX reference dye are satisfactory for the measurement of the Reference Dye for Quantitative PCR. A tube of 25 mM MgCl2 is also provided for easy optimization of the qPCR reaction.

Default reaction volume is 50 μL

20RXN is packaged as 1 X 500 μL
100RXN is packaged as 1 X 2.5 mL
400RXN is packaged as 1 X 10 mL

キットの構成要素は別途購入可能です。

製品番号
詳細
SDS
  • P2893JumpStart™ Taq ReadyMix™, Complete optimized reagent for hot-start PCR at 2X concentration 2 XSDS
  • M878725 mM MgCl2 1.5 μLSDS
  • R4526Reference Dye for Quantitative PCR, 100 ×, solution 100 XSDS

pictograms

Exclamation markEnvironment
GHS07,GHS09

signalword

Warning

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

保管分類

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

最新バージョンのいずれかを選択してください：

試験成績書（COA）

Lot/Batch Number
0000517871
0000517875
0000517875
0000517871
0000483236

適切なバージョンが見つかりませんか。

特定のバージョンが必要な場合は、ロット番号またはバッチ番号で特定の証明書を検索できます。

COAを検索

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Eleonora De Martino et al.
Cancers, 12(10) (2020-10-03)
Cutaneous melanoma (CM) incidence is rising worldwide and is the primary cause of death from skin disease in the Western world. Personal risk factors linked to environmental ultraviolet radiation (UVR) are well-known etiological factors contributing to its development. Nevertheless, UVR
J G Schmidt et al.
Fish & shellfish immunology, 48, 273-284 (2015-12-26)
We followed the progression of healing of deep excisional biopsy punch wounds over the course of 365 days in rainbow trout (Oncorhynchus mykiss) by monitoring visual wound healing and gene expression in the healing muscle at regular intervals (1, 3
Caroline T Weldon et al.
PLoS neglected tropical diseases, 12(8), e0006708-e0006708 (2018-08-31)
Zika virus was reported in the rainforest city of Iquitos, Peru in 2016. The potential associations between Zika and fetal neurological disorders were reported extensively in the media regarding neighboring Brazil, and led to great concern about the impact Zika
Lithium, an anti-psychotic drug, greatly enhances the generation of induced pluripotent stem cells.
Wang, Q., et al.
Cell Research (2011)
Benjamin B Gelman et al.
Journal of acquired immune deficiency syndromes (1999), 62(5), 487-495 (2012-12-18)
Replicating HIV-1 in the brain is present in HIV encephalitis (HIVE) and microglial nodule encephalitis (MGNE) and is putatively linked with HIV-associated neurocognitive disorders (HAND). A cliniconeurovirological correlation was conducted to elucidate the relationship between brain viral load and clinical
関連する文献をすべて表示

資料

Polymerase Chain Reaction

The polymerase chain reaction is one of the most widely used techniques in molecular biology. The PCR process consists of three main steps, Denaturation, Annealing & Extension

Probe-based qPCR

Probe-based QPCR offers high sensitivity and specificity with fluorescent-labeled probes and various dye options for multiplex amplification.

Probe QPCR for Gene-Level Expression

qPCR investigates gene expression, amplification, and alterations, crucial for tumor biology and understanding cancer genetics.

Hot Start PCR

The purpose of Hot Start PCR is to inhibit the PCR reaction in order to reduce nonspecific amplification, prevent the formation of primer dimers, and increase product yields.

すべて表示

関連コンテンツ

RT-qPCR – Quantitative Reverse Transcription PCR

RT-qPCR detects specific targets with applications in gene expression and pathogen detection.

RT-qPCR – 定量的逆転写PCR

RT-qPCRは、遺伝子発現や病原体検出における用途で、特定のターゲットを検出します。

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

製品に関するお問い合わせはこちら（テクニカルサービス）