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M6018

4-Methylumbelliferyl β-D-cellobioside

glucanase substrate, fluorogenic, ≥98.00% (TLC), powder

Synonym(s):

4-Methylumbelliferyl beta-D-cellobioside

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About This Item

Empirical Formula (Hill Notation):
C22H28O13
CAS Number:
72626-61-0
Molecular Weight:
500.45
NACRES:
NA.32
PubChem Substance ID:
24897067
UNSPSC Code:
12352204
EC Number:
276-744-7
MDL number:
MFCD00063279
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Product Name

4-Methylumbelliferyl β-D-cellobioside, glucanase substrate

Quality Level

200

assay

≥98.00% (TLC)

form

powder

solubility

water: 19.60-20.40 mg/mL, clear, colorless to faintly yellow

storage temp.

2-8°C

SMILES string

CC1=CC(=O)Oc2cc(O[C@@H]3O[C@H](CO)[C@@H](O[C@@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@H]4O)[C@H](O)[C@H]3O)ccc12

InChI

1S/C22H28O13/c1-8-4-14(25)32-11-5-9(2-3-10(8)11)31-21-19(30)17(28)20(13(7-24)34-21)35-22-18(29)16(27)15(26)12(6-23)33-22/h2-5,12-13,15-24,26-30H,6-7H2,1H3/t12-,13-,15-,16+,17-,18-,19-,20-,21-,22+/m1/s1

InChI key

PRTGXBPFDYMIJH-MKQZUAMYSA-N

Application

4-Methylumbelliferyl β-D-cellobioside has been used as a substrate:
  • to assay cellulase from A. niger
  • to determine the extracellular enzyme activity for 1,4-β-cellobiohydrolase
  • to assay the activity of bound enzyme, endoglucanase I (EGIc) Trichoderma reesei class II hydrophobins (HFBI)

Biochem/physiol Actions

4-Methylumbelliferyl β-D-cellobioside is used as a substrate for cellulase assays. It is mainly used for activity staining of cellulases in polyacrylamide gels.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000430619
0000551746
0000551747
0000226092
0000453971

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Related Content

Product Information Sheet - M6018

Product Information Sheet

M Pedraza-Reyes et al.
Archives of microbiology, 168(4), 321-327 (1997-09-23)
The gram-negative bacterium Myxobacter sp. AL-1 produces chitosanase-cellulase activity that is maximally excreted during the stationary phase of growth. Carboxymethylcellulase zymogram analysis revealed that the enzymatic activity was correlated with two bands of 32 and 35 kDa. Ion-exchange-chromatography-enriched preparations of
A V Gusakov et al.
Biochimica et biophysica acta, 1073(3), 481-485 (1991-04-09)
Using 4-methylumbelliferyl (MUF) beta-D-cellobioside as a substrate, the ability of cellobiohydrolase I from Trichoderma longibrachiatum to catalyze transglycosylation has been demonstrated. At substrate concentrations greater than 2 mM, the formation of MUF-tetrasaccharide was detected using HPLC. In the course of
K H Jung et al.
Biochemistry and molecular biology international, 44(2), 283-292 (1998-04-08)
A Clostridium thermocellum xylanase gene, designated xynX, was cloned in Escherichia coli and was categorized a novel gene as a result of the comparison of restriction patterns of the C. thermocellum xylanase genes so far reported. The xynX gene encodes
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