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MF-Millipore™ Membrane Filters

Mixed cellulose esters

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    MF-Millipore Filters: A biologically inert mixture of cellulose acetate and cellulose nitrate

    Referencias bibliográficas

    Visión general referenciasAplicación
    Mobile Phase Preparation for UHPLC: Membrane Filtration Method Affects System Performance and Leaching of Extractable Impurities
    Subodh Kulkarn(1), Jesmi George(2) and Vivek Joshi(2) (1) Millipore India Pvt. Ltd., Bioscience Division, 50A, 2nd Phase, Ring Road, Peenya, Bangalore, India 560058 (2) Millipore Corp., Bioscience Division, 17 Cherry Hill Drive, Danvers, MA 01923
    LCGC 2010

    Mostrar resumen Artículo Texto completo
    Direct Effect of Vanadium on Citrate Uptake by Rat Renal Brush Border Membrane Vesicles (BBMV)
    Kazuhiro Sato, et al.
    Industrial Health , 40, 278-281, 2002, internal ref BB02 2002

    Effect of Platinum Coordination Complex (PtCx) on Citrate Uptake by Rat Renal Bush Border Membrane vesicles (BBMV):Direct Effect of Carboplatin
    Kazuhiro Sato, et al.
    Industrial Health, 39, 21-31, 2001; internal ref BB01 2001

    A sensitive Elispot assay to detect low-frequency human T-lymphocytes
    McCutcheon M, Wehner N, Wensky A et al.
    J Immunol Methods 210(2): 149-166. 1997

    ELISPOT Assays
    Direct effect of cadmium on citrate uptake by isolated rat renal brush border membrane cesicles
    Kazuhiro Sato, et al.
    Toxicology Letters, 80, 161-165, 1995, internal ref BB06 1995

    Stoichiometry of Na+-Succinate Cotransport in Renla Brush-Border Membranes
    Stephen H. at al.,Journal of Biological Chemistry, 257, 4, 1773-1778, 1982, Internal ref bb03
    Journal of Biological Chemistry, 257, 4, 1773-1778, 1982, Internal ref bb03 1982

    Preguntas frecuentes

    PreguntaRespuesta
    What would you recommend to dissolve MF ( mixed cellulose ester ) filters? Many solvents are possible. A popular choice is acetone, which is non-toxic and highly volatile so it is easy to evaporate (but quite a flammable). Another choice is NMP (n-methylpyrrolidinone), which is not very volatile at room temperature. The selection is often based on what is to be done after the dissolution.
    How do I clear my Millipore filters? It is possible to clear Millipore filters by choosing a clearing agent with the same refractive index as the membrane. This is called the PORE FILLING TECHNIQUE. Filters appear opaque because of the diffraction of light through the tortuous pores. Filling the pores with a liquid that has the same refractive index as the membrane (ex. xylene for MF filters) allows light to pass through the filter at a uniform speed thus rendering the filter transparent. This technique can be used for most membrane filters with a single refractive index. The refractive index for Durapore membrane is 1.42 and for MF membrane its 1.50.

    Please note that this does not work for polycarbonate membranes because these membranes have more than one refractive index (1.62 and 1.58). If desired, the porous structure can be dissolved from Isopore polycarbonates by dissolving the filter in chloroform, methylene chloride or 1-methyl-2 pyrrolidone.
    What is the blue separation paper between my filters? It is a parchmentalized paper specially formulated for Millipore Corporation.
    How do I tell the membrane from the separator? The blue paper is the separator.
    How do I assemble a diffusion chamber containing tissue for implantation?
    1. Wet a single membrane filter (either GSWP 013 00 or HAWP 013 00) with Milli-Q water and blot it to absorbent paper to remove excess water.
    2. Glue the filter to one side of a diffusion chamber ring without hole (catalog number PR00 014 00), using MF cement (catalog number XX70 000 00), and let dry.
    3. Sterilize this assembly, along with another membrane filter of the same type, by exposure to ethylene oxide gas or ultraviolet light.
    4. Moisten the half-completed chamber and the separate sterile filter with a sterile physiological solution, and aseptically place the tissue to be studied in the half-completed chamber assembly.
    5. Glue the cover filter in place using sterile technique.
    What are the dimensions and width of the grids on the Millipore 47mm gridded membranes? The grids are 3.08mm x 3.08mm square. A grid line is 44um in width and there is are average of 169 gridded squares per filter. Please note however that the actual filtration area and therefore the total number of squares in that area is dependent on the filter holder. For filter holders with 9.6sq.cm of filtration area there are 100 squares available. For filter holders with 13.8 sq.cm of filtration area the number of available grids are approximately 140.
    How do I assemble a diffusion chamber containing suspension cells for implantation? Wet two membrane filters (either GSWP 013 00 or HAWP 013 00) with Milli-Q water and blot them to absorbent paper to remove excess water. Glue a moist filter to each side of the diffusion chamber ring with hole (catalog number PR00 014 01) using MF cement (catalog number XX70 000 00). Sterilize the dried diffusion chamber with ethylene oxide gas or ultraviolet light. After sterilization, inject the cells through the hole in the plastic ring, and plug the hole with the nylon thread which comes with the rings (catalog number PR00 000 00 if ordered separately).
    Which membrane should I use to filter my solution? For general filtration use MCE. When you are looking for the lowest protein binding membrane use Durapore. For speed, and when filtering serum solutions use Millipore Express.
    Which side of the membrane should I use, the shiny or dull side? Most researchers may not even notice that there is a "sidedness" to filters, and, for most applications, orientation will not affect filter performance. However, membranes do have a slightly asymmetric pore structure: the shiny side of the membrane is the "tighter" side. In some applications, you can take advantage of this difference by selecting a specific filter orientation. ultrafiltration membranes should always be used shiny side up, regardless of application for drop dialysis ( a buffer exchange technique in which a few drops of DNA or protein are placed in a 0.05 or 0.025 um filter and floated on a buffer solution), apply the sample to the shiny side of the filter and float the filter dull side to the buffer. This measure will enhance buffer exchange and discourage sample loss. The Millipore Express and Express Plus membranees are also sided - these membranes should be used shiny side facing down.
    Can I autoclave my filters? Millipore's MF, Durapore, Fluoropore and Mitex filters are all autoclavable. Standard conditions of 121-126C for 20-30 min generally work quite well. However, the filters should be autoclaved on a "slow exhaust (liquid) cycle. Because Fluoropore membranes have a polyethylene backing they will be slightly wrinkled after autoclaving.
    What filter do you recommend for dialyzing 5-100ul sample volumes of proteins and nucleic acids (i.e. to perform a drop dialysis technique)? With the Millipore drop dialysis technique 0.025 um or 0.05 um MF mixed cellulose ester membranes are commonly used. This simple- to-use technique takes advantage of the varying diffusion rates of different size molecules to quickly dialyze small volume samples. The technique involves filling the bottom of a Petri dish with dialysate and floating the membrane filter disc, shiny side up, on the surface of the solution. You then deposit 5-100 ul samples on the center of the disc with a micropipet. use of dye markers in the sample may help to determine the dialysis time required. Most samples are dialyzed in less than 30 minutes. then simply recover the desalted sample with a micropipet.