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Anti-Clr-a clone 2E3, Cat. No. MABF2209, is a rat monoclonal antibody that detects C-type lectin domain family 2 member E and is tested in Flow Cytometry, Immunohistochemistry, and Western Blotting.
More>>Anti-Clr-a clone 2E3, Cat. No. MABF2209, is a rat monoclonal antibody that detects C-type lectin domain family 2 member E and is tested in Flow Cytometry, Immunohistochemistry, and Western Blotting. Less<<
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Übersicht
Replacement Information
Description
Catalogue Number
MABF2209-100UL
Description
Anti-Clr-a Antibody, clone 2E3
Alternate Names
C-type lectin domain family 2 member E
C-type lectin-related protein A
Background Information
C-type lectin domain family 2 member E (UniProt: I1W6X0; also known as C-type lectin-related protein A, Clr-a) is encoded by the Clec2e (also known as Clra) gene (Gene ID: 232409) in murine species. Clr-a is an NKC-encoded, disulfide-linked homodimeric, single-pass type II membrane protein glycoprotein that is shown to be exclusively expressed by mouse gut epithelium. Hence, it can be used as a marker for intestinal epithelial cells (IECs). Its expression has been reported at the cell surface on the apical as well as basolateral side of the IECs. Clr-a has an N-terminal cytoplasmic domain followed by a transmembrane domain, and an extracellular domain containing C-type lectin-like domain. It has been shown that a single amino acid exchange at position 35 (A35G) results in a substantially higher expression of Clr-a. Clone 2E3 specifically detects Clr-a on epithelial cells and targets an epitope (LTRGGGECAY) in the extracellular domain. (Ref.: Rutkowski, E., et al. (2017). J. Immunol. 198(2); 916-926).
References
Product Information
Format
Purified
Presentation
Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application
Anti-Clr-a clone 2E3, Cat. No. MABF2209, is a rat monoclonal antibody that detects C-type lectin domain family 2 member E and is tested in Flow Cytometry, Immunohistochemistry, and Western Blotting.
Key Applications
Immunohistochemistry
Western Blotting
Application Notes
Western Blotting Analysis A representative lot detected Clr-a in Western Blotting applications (Ruthkowski, E., et. al. (2017). J Immunol. 198(2); 916-926).
Flow Cytometry Analysis: A representative lot detected Clr-a in Flow Cytometry applications (Ruthkowski, E., et. al. (2017). J Immunol. 198(2); 916-926).
Immunohistochemistry Analysis: A representative lot detected Clr-a in Immunohistochemistry applications (Ruthkowski, E., et. al. (2017). J Immunol. 198(2); 916-926).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
Immunogen
c-Myc/His-tagged recombinant fragment corresponding to 140 amino acids from the extracellular domain of murine C-type lectin domain family 2 member E (Clr-a).
Epitope
extracellular domain
Clone
2E3
Concentration
0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Host
Rat
Specificity
Clone 2E3 is a rat monoclonal antibody that detects murine C-type lectin domain family 2 member E. It targets an epitope within 10 amino acids from the C-terminal extracellular domain.
~26 kDa observed; 24.00 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in mouse small intestine tissue lysate.
Western Blotting Analysis: A 1:250 dilution of this antibody detected Clr-a in mouse small intestine tissue lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Clr-a: A Novel Immune-Related C-Type Lectin-like Molecule Exclusively Expressed by Mouse Gut Epithelium. Rutkowski E, Leibelt S, Born C, Friede ME, Bauer S, Weil S, Koch J, Steinle A. J Immunol
198(2)
916-926
2016
The mouse gut epithelium represents a constitutively challenged environment keeping intestinal commensal microbiota at bay and defending against invading enteric pathogens. The complex immunoregulatory network of the epithelial barrier surveillance also involves NK gene complex (NKC)-encoded C-type lectin-like molecules such as NKG2D and Nkrp1 receptors. To our knowledge, in this study, we report the first characterization of the orphan C-type lectin-like molecule Clr-a encoded by the Clec2e gene in the mouse NKC. Screening of a panel of mouse tissues revealed that Clec2e transcripts are restricted to the gastrointestinal tract. Using Clr-a-specific mAb, we characterize Clr-a as a disulfide-linked homodimeric cell surface glycoprotein. Of note, a substantial fraction of Clr-a molecules are retained intracellularly, and analyses of Clr-a/Clr-f hybrids attribute intracellular retention to both the stalk region and parts of the cytoplasmic domain. Combining quantitative PCR analyses with immunofluorescence studies revealed exclusive expression of Clr-a by intestinal epithelial cells and crypt cells throughout the gut. Challenge with polyinosinic-polycytidylic acid results in a rapid and strong downregulation of intestinal Clr-a expression in contrast to the upregulation of Clr-f, a close relative of Clr-a, that also is specifically expressed by the intestinal epithelium and acts as a ligand of the inhibitory Nkrp1g receptor. Collectively, we characterize expression of the mouse NKC-encoded glycoprotein Clr-a as strictly associated with mouse intestinal epithelium. Downregulation upon polyinosinic-polycytidylic acid challenge and expression by crypt cells clearly distinguish Clr-a from the likewise intestinal epithelium-restricted Clr-f, pointing to a nonredundant function of these highly related C-type lectin-like molecules in the context of intestinal immunosurveillance.
