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MABF1105-25UG Anti-DAG Kinase Alpha Antibody, clone 10D6.1

This mouse monoclonal Anti-DAG Kinase Alpha, clone 10D6.1, Cat. No. MABF1105-25UG is validated for use in Western Blotting and Immunohistochemistry (Paraffin), for the detection of DGKA.

Anti-DAG Kinase Alpha Antibody, clone 10D6.1: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Research Category: Inflammation & Immunology Gene Symbol: DGKA, DAGK, DAGK1 UniProt Number: P23743
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MABF1105-25UG
25 μg  
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      Übersicht

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABF1105-25UG
      DescriptionAnti-DAG Kinase Alpha Antibody, clone 10D6.1
      Alternate Names
      • Diacylglycerol kinase alpha
      • 80 kDa diacylglycerol kinase
      • Diglyceride kinase alpha
      • DGK-alpha
      Background InformationDiacylglycerol kinase alpha (UniProt: P23743; also known as EC:2.7.1.107, DAG kinase alpha, 80 kDa diacylglycerol kinase, Diglyceride kinase alpha, DGK-alpha) is encoded by the DGKA (also known as DAGK, DAGK1) gene (Gene ID: 1606) in human. Diacylglycerol kinase is a lipid kinase that converts diacylglycerol to phosphatidic acid, and regulates the activity of many enzymes including protein kinase C, phosphatidylinositol 4-phosphate 5-kinase, and mTOR. Thus far ten mammalian subtypes of DAG kinase have been reported. All DAG kinase with the exception of beta isoform are localized in cytoplasm in several cells, but some translocate to the plasma membrane upon activation. DAG kinase acts as a modulator, which competes with protein kinase C for diacylglycerol in intracellular signaling pathways. DAG kinase also plays a role in the resynthesis of phosphatidylinositols and phosphorylating diacylglycerol to phosphatidic acid. Most DAG kinases, with the exception of theta, contain two cysteine-rich regions (C1A and C1B domains) in the regulatory domain of the N-terminal half of the molecule. In DAK kinase alpha the C1 domain closest to the catalytic domain has an extended region of fifteen amino acids that is not present in C1 domains of other DAG kinases. DAG kinase alpha can be activated by the phosphorylation of Tyr335, which occurs during T cell activation or following exposure of cells to hepatocyte growth factor.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationThis mouse monoclonal Anti-DAG Kinase Alpha, clone 10D6.1, Cat. No. MABF1105-25UG is validated for use in Western Blotting and Immunohistochemistry (Paraffin), for the detection of DGKA.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected DAG Kinase Alpha in human tonsil and human breast cancer tissue.
      Biological Information
      ImmunogenGST/His-tagged recombinant fragment corresponding to the first 110 amino acids of human DAG kinase alpha.
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 10D6.1 detects DAG kinase alpha in human. It targets an epitope within the first 110 amino acids from the N-terminal region.
      IsotypeIgG2a
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • DGKA
      • DAGK
      • DAGK1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight83 kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Jurkat cell lysate.
      Western Blotting Analysis:  1 µg/mL of this antibody detected DAG Kinase Alpha in 10 µg of Jurkat cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      MABF1105-25UG 04054839456213