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Die folgenden MAPmates™ sollten nicht zusammen analysiert werden: -MAPmates™, die einen unterschiedlichen Assaypuffer erfordern. -Phosphospezifische und MAPmate™ Gesamtkombinationen wie Gesamt-GSK3β und Gesamt-GSK3β (Ser 9). -PanTyr und locusspezifische MAPmates™, z.B. Phospho-EGF-Rezeptor und Phospho-STAT1 (Tyr701). -Mehr als 1 Phospho-MAPmate™ für ein einziges Target (Akt, STAT3). -GAPDH und β-Tubulin können nicht mit Kits oder MAPmates™, die panTyr enthalten, analysiert werden.
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48-602MAG
Buffer Detection Kit for Magnetic Beads
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Anti-IFIT1, clone 13B4-1, Cat. No. MABF2228, is a highly specific mouse monoclonal antibody that targets Interferon-induced protein with tetratricopeptide repeats 1(IFIT1) and has been tested for use in Western Blotting.
More>>Anti-IFIT1, clone 13B4-1, Cat. No. MABF2228, is a highly specific mouse monoclonal antibody that targets Interferon-induced protein with tetratricopeptide repeats 1(IFIT1) and has been tested for use in Western Blotting. Less<<
Interferon-induced protein with tetratricopeptide repeats 1
IFIT-1
Glucocorticoid-attenuated response gene 16 protein
GARG-16
Interferon-induced 56 kDa protein
IFI-56K
P56
Background Information
Interferon-induced protein with tetratricopeptide repeats 1 (UniProt: Q64282; also known as IFIT-1, Glucocorticoid-attenuated response gene 16 protein, GARG-16, Interferon-induced 56 kDa protein, IFI-56K, P56) is encoded by the Ifit1 (also known as Garg16, Ifi56, Isg56) in human. IFIT1 is a component of an interferon-dependent multiprotein complex along with IFIT2 and IFIT3. It is an interferon-induced antiviral RNA-binding protein that specifically binds single-stranded RNA bearing a 5'-triphosphate group (PPP-RNA), thereby acting as a sensor of viral single-stranded RNAs and inhibiting expression of viral messenger RNAs. It can exist both in homo- and heterooligomeric forms. IFIT1 contains 10 tetratricopeptide repeats (TPRs) that form globular N- and C-terminal domains joined by a linker of variable flexibility. Its RNA recognition is mediated by a convoluted intramolecular fold of the TPR repeats, which scaffolds unique additional helices that form an RNA binding cleft. IFIT1 inhibits translation by binding directly to the 5' end of foreign RNAs, particularly those with non-self-cap structures, precluding the recruitment of the cap-binding eukaryotic translation initiation factor 4F and ribosome recruitment. The presence of IFIT1 imposes a requirement on viruses that replicate in the cytoplasm to maintain mechanisms to avoid its restrictive effects. (Ref.: Fleith, RC., et al. (2018). Nucleic Acids Res. 46(10); 5269-5285; Daniels, BP., et al. (2017). J. Clin. Invest. 127(3); 843-856).
References
Product Information
Format
Purified
Presentation
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application
Anti-IFIT1, clone 13B4-1, Cat. No. MABF2228, is a highly specific mouse monoclonal antibody that targets Interferon-induced protein with tetratricopeptide repeats 1(IFIT1) and has been tested for use in Western Blotting.
Key Applications
Western Blotting
Application Notes
Western Blotting Analysis: A representative lot detected IFIT1 in Western Blotting applications (Daniels, B.P., et. al. (2017). J Clin Invest. 127(3):843-856).
Biological Information
Immunogen
His-tagged full-length recombinant mouse Interferon-induced protein with tetratricopeptide repeats 1 (ITIT1).
Clone
13B4-1
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Specificity
Clone 13B4-1 specifically detects murine Interferon-induced protein with tetratricopeptide repeats 1 (IFIT1).
~53 kDa observed; 53.74 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in mouse lung tissue lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected IFIT1 in mouse lung tissue lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Type I IFNs promote cellular responses to viruses, and IFN receptor (IFNAR) signaling regulates the responses of endothelial cells of the blood-brain barrier (BBB) during neurotropic viral infection. However, the role of astrocytes in innate immune responses of the BBB during viral infection of the CNS remains to be fully elucidated. Here, we have demonstrated that type I IFNAR signaling in astrocytes regulates BBB permeability and protects the cerebellum from infection and immunopathology. Mice with astrocyte-specific loss of IFNAR signaling showed decreased survival after West Nile virus infection. Accelerated mortality was not due to expanded viral tropism or increased replication. Rather, viral entry increased specifically in the hindbrain of IFNAR-deficient mice, suggesting that IFNAR signaling critically regulates BBB permeability in this brain region. Pattern recognition receptors and IFN-stimulated genes had higher basal and IFN-induced expression in human and mouse cerebellar astrocytes than did cerebral cortical astrocytes, suggesting that IFNAR signaling has brain region-specific roles in CNS immune responses. Taken together, our data identify cerebellar astrocytes as key responders to viral infection and highlight the existence of distinct innate immune programs in astrocytes from evolutionarily disparate regions of the CNS.
