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Anti-KCNK9, clone Y4, Cat. No. MABC1743, is a mouse monoclonal antibody that detects Potassium channel subfamily K member 9 and is tested for use in Flow Cytometry, Function Analysis, Inhibition Assay, and Western Blotting.
More>>Anti-KCNK9, clone Y4, Cat. No. MABC1743, is a mouse monoclonal antibody that detects Potassium channel subfamily K member 9 and is tested for use in Flow Cytometry, Function Analysis, Inhibition Assay, and Western Blotting. Less<<
Anti-Low Density Lipoprotein Antibody, HOCL oxidized
Übersicht
Replacement Information
Description
Catalogue Number
MABC1743-100UL
Description
Anti-KCNK9 Antibody, clone Y4
Alternate Names
Potassium channel subfamily K member 9
Acid-sensitive potassium channel protein TASK-3
TWIK-related acid-sensitive K(+) channel 3
Two pore potassium channel KT3.2
Two pore K(+) channel KT3.2
Background Information
Potassium channel subfamily K member 9 (UniProt: Q9NPC2; also known as Acid-sensitive potassium channel protein TASK-3, TWIK-related acid-sensitive K(+) channel 3, Two pore potassium channel KT3.2, Two pore K(+) channel KT3.2, KCNK9) is encoded by the KCNK9 (also known as TASK3) gene (Gene ID: 51305) in human. KCNK9 is a homodimeric multi-pass membrane protein of the two-pore domain potassium channel that maintains resting membrane potential of the cell. It is highly sensitive to changes in pH. It can also form heterodimer with KCNK1. It is found mainly in cerebellum, but has also been detected in adrenal gland, kidney, and lung. Over-expression of KCNK9 has been reported in several carcinomas, including breast, lung, colon, and prostate. It is reported to confer resistance to growth factor deprivation and hypoxia, which promotes tumor cell survival in hypoxic areas of solid tumors. KCNK9 has three cytoplasmic domains (aa 1-8, 129-158, and 240-374), four transmembrane domains, four extracellular domains, and two pore-forming domains (aa 89-101 and 195-207). Clone Y4 displays highest affinity for KCNK9 (KD = 0.7 nM) and induces channel internalization. Addition of Y4 to KCNK9-expressing carcinoma cells is shown to reduce their viability and increase cell death. It also inhibits the growth of human lung cancer xenografts and murine breast cancer metastasis in mice. (Ref.: Sun, H., et al. (2016). Nat. Commun. 7; 10339).
References
Product Information
Format
Purified
Presentation
Purified mouse monoclonal antibody IgG1 in PBS without azide
Anti-KCNK9, clone Y4, Cat. No. MABC1743, is a mouse monoclonal antibody that detects Potassium channel subfamily K member 9 and is tested for use in Flow Cytometry, Function Analysis, Inhibition Assay, and Western Blotting.
Key Applications
Flow Cytometry
Function Assay
Inhibition
Western Blotting
Application Notes
Western Blotting Analysis: A representative lot detected KCNK9 in Western Blotting applications (Sun, H., et. al. (2016). Nat Commun. 7:10339).
Flow Cytometry Analysis: 10 ug per million cells from a representative lot detected KCNK9 in BEN cells (Courtesy of John Laterra, M.D., Ph.D., Kennedy Krieger Institute, John Hopkins Hospital, Baltimore, MD, USA).
Inhibits Activity/Function: A representative lot inhibited growth of human lung cancer xenografts and murine breast cancer metastasis in mice. (Sun, H., et. al. (2016). Nat Commun. 7:10339).
Flow Cytometry Analysis: A representative lot detected KCNK9 in Flow Cytometry applications (Sun, H., et. al. (2016). Nat Commun. 7:10339).
Affects Function: A representative lot induced endocytosis of functional channels. (Sun, H., et. al. (2016). Nat Commun. 7:10339).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
Immunogen
Recombinant fragment corresponding to the 59 amino acids from the first extracellular domain (M1P1 loop) of human Potassium channel subfamily K member 9 (KCNK9).
Epitope
extracellular domain
Clone
Y4
Concentration
0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Host
Mouse
Specificity
Clone Y4 is a mouse monoclonal antibody that detects human Potassium channel subfamily K member 9 (KCNK9). It targets an epitope within 59 amino acids from the first extracellular loop.
Flow Cytometry Analysis: A 1:50 dilution of this antibody detected KCNK9 in one million BT-549 cells.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -10°C to -25°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
A monoclonal antibody against KCNK9 K(+) channel extracellular domain inhibits tumour growth and metastasis Han Sun 1 2 , Liqun Luo 3 , Bachchu Lal 2 , Xinrong Ma 4 , Lieping Chen 5 , Christine L Hann 6 , Amy M Fulton 4 7 , Daniel J Leahy 8 , John Laterra 1 2 6 9 , Min Li Nat Commun
7
10339
2015
Two-pore domain potassium (K2P) channels act to maintain cell resting membrane potential--a prerequisite for many biological processes. KCNK9, a member of K2P family, is implicated in cancer, owing to its overexpression in human tumours and its ability to promote neoplastic cell survival and growth. However, KCNK9's underlying contributions to malignancy remain elusive due to the absence of specific modulators. Here we describe the development of monoclonal antibodies against the KCNK9 extracellular domain and their functional effects. We show that one antibody (Y4) with the highest affinity binding induces channel internalization. The addition of Y4 to KCNK9-expressing carcinoma cells reduces cell viability and increases cell death. Systemic administration of Y4 effectively inhibits growth of human lung cancer xenografts and murine breast cancer metastasis in mice. Evidence for Y4-mediated carcinoma cell autonomous and immune-dependent cytotoxicity is presented. Our study reveals that antibody-based KCNK9 targeting is a promising therapeutic strategy in KCNK9-expressing malignancies.
