Wenn Sie das Fenster schließen, wird Ihre Konfiguration nicht gespeichert, es sei denn, Sie haben Ihren Artikel in die Bestellung aufgenommen oder zu Ihren Favoriten hinzugefügt.
Klicken Sie auf OK, um das MILLIPLEX® MAP-Tool zu schließen oder auf Abbrechen, um zu Ihrer Auswahl zurückzukehren.
Wählen Sie konfigurierbare Panels & Premixed-Kits - ODER - Kits für die zelluläre Signaltransduktion & MAPmates™
Konfigurieren Sie Ihre MILLIPLEX® MAP-Kits und lassen sich den Preis anzeigen.
Konfigurierbare Panels & Premixed-Kits
Unser breites Angebot enthält Multiplex-Panels, für die Sie die Analyten auswählen können, die am besten für Ihre Anwendung geeignet sind. Unter einem separaten Register können Sie das Premixed-Cytokin-Format oder ein Singleplex-Kit wählen.
Kits für die zelluläre Signaltransduktion & MAPmates™
Wählen Sie gebrauchsfertige Kits zur Erforschung gesamter Signalwege oder Prozesse. Oder konfigurieren Sie Ihre eigenen Kits mit Singleplex MAPmates™.
Die folgenden MAPmates™ sollten nicht zusammen analysiert werden: -MAPmates™, die einen unterschiedlichen Assaypuffer erfordern. -Phosphospezifische und MAPmate™ Gesamtkombinationen wie Gesamt-GSK3β und Gesamt-GSK3β (Ser 9). -PanTyr und locusspezifische MAPmates™, z.B. Phospho-EGF-Rezeptor und Phospho-STAT1 (Tyr701). -Mehr als 1 Phospho-MAPmate™ für ein einziges Target (Akt, STAT3). -GAPDH und β-Tubulin können nicht mit Kits oder MAPmates™, die panTyr enthalten, analysiert werden.
.
Bestellnummer
Bestellinformationen
St./Pkg.
Liste
Dieser Artikel wurde zu Ihren Favoriten hinzugefügt.
Wählen Sie bitte Spezies, Panelart, Kit oder Probenart
Um Ihr MILLIPLEX® MAP-Kit zu konfigurieren, wählen Sie zunächst eine Spezies, eine Panelart und/oder ein Kit.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
Catalogue Number
Ordering Description
Qty/Pack
List
Dieser Artikel wurde zu Ihren Favoriten hinzugefügt.
Spezies
Panelart
Gewähltes Kit
Menge
Bestellnummer
Bestellinformationen
St./Pkg.
Listenpreis
96-Well Plate
Menge
Bestellnummer
Bestellinformationen
St./Pkg.
Listenpreis
Weitere Reagenzien hinzufügen (MAPmates erfordern die Verwendung eines Puffer- und Detektionskits)
Menge
Bestellnummer
Bestellinformationen
St./Pkg.
Listenpreis
48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Platzsparende Option Kunden, die mehrere Kits kaufen, können ihre Multiplex-Assaykomponenten in Kunststoffbeuteln anstelle von Packungen erhalten, um eine kompaktere Lagerung zu ermöglichen.
Dieser Artikel wurde zu Ihren Favoriten hinzugefügt.
Das Produkt wurde in Ihre Bestellung aufgenommen
Sie können nun ein weiteres Kit konfigurieren, ein Premixed-Kit wählen, zur Kasse gehen oder das Bestell-Tool schließen.
Anti-MICA, clone AMO-1, Cat. No. MABF2220, is a mouse monoclonal antibody that detects MHC class I polypeptide-related sequence A (MICA) and is tested for use in ELISA, Flow Cytometry, and Inhibition Assay.
More>>Anti-MICA, clone AMO-1, Cat. No. MABF2220, is a mouse monoclonal antibody that detects MHC class I polypeptide-related sequence A (MICA) and is tested for use in ELISA, Flow Cytometry, and Inhibition Assay. Less<<
Empfohlene Produkte
Übersicht
Replacement Information
Description
Catalogue Number
MABF2220-25UG
Description
Anti-MICA Antibody, clone AMO-1
Alternate Names
MHC class I polypeptide-related sequence A
MIC-A
Background Information
MHC class I polypeptide-related sequence A (UniProt: Q29983; also known as MICA, MIC-A) is encoded by the MICA (also known as PERB11.1) gene (Gene ID: 100507436) in human. MICA is a single-pass type I membrane glycoprotein that acts as stress-induced self-antigen. It serves as a ligand for the KLRK1/NKG2D receptor. It is recognized by cytotoxic gamma-delta T cells expressing the TCR variable region V delta1. MICA is widely expressed with the exception of the central nervous system. It is predominant in gastric epithelium and in monocytes, keratinocytes, endothelial cells, and fibroblasts. It is also expressed in tumors of epithelial origin. A soluble form of MICA (sMICA) has also been described that is released from the cell surface of tumor cells following proteolytic cleavage and can be detected in serum of subjects with gastrointestinal malignancies. This can impair KLRK1/NKG2D expression and T-cell activation. MICA can be induced by heat shock and by exposure to DNA damaging conditions. It is synthesized with a signal peptide (aa 1-23), which is subsequently cleaved off in the mature form. Their mature forms contain an extracellular domain (aa 24-304), a transmembrane domain (aa 308-328), and a cytoplasmic domain (aa 329-383) (Ref.: Groh, V., et al. (1999). Proc. Natl. Acad. Sci USA. 96 (12); 6879-6884; Salih, HR et al. (2003). Blood. 102(4); 1389-1396).
References
Product Information
Format
Purified
Presentation
Purified mouse monoclonal antibody IgG1 in PBS without azide.
Anti-MICA, clone AMO-1, Cat. No. MABF2220, is a mouse monoclonal antibody that detects MHC class I polypeptide-related sequence A (MICA) and is tested for use in ELISA, Flow Cytometry, and Inhibition Assay.
Key Applications
ELISA
Flow Cytometry
Inhibition
Application Notes
Flow Cytometry Analysis: A 1:500 dilution from a representative lot detected MICA in C1R-MICA cell transfectants (Courtesy of Dr. Alexander Steinle, Institute for Molecular Medicine, Goethe-University Frankfurt am Main, Germany).
Flow Cytometry Analysis: A representative lot detected MICA in Flow Cytometry applications (Welte, S.A., et. al. (2003). Eur J Immunol. 33(1):194-203; Salih, H.R., et. al. (2003). Blood. 102(4):1389-96).
Inhibition Analysis: A representative lot Inhibited the interaction between MICA and NKG2D in leukemic cells. (Salih, H.R., et. al. (2003). Blood. 102(4):1389-96).
ELISA Analysis: A representative lot detected MICA in ELISA applications (Salih, H.R., et. al. (2003). Blood. 102(4):1389-96).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
Immunogen
Mixture of full-length MICA01 and MICA04 expressed in mouse P815 cell line.
Epitope
extracellular domain
Clone
AMO-1
Concentration
1 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Host
Mouse
Specificity
Clone AM0-1 is a mouse monoclonal antibody that detects human MHC class I polypeptide-related sequence A (MICA). It targets an epitope within the extracellular domain.
Flow Cytometry Analysis: A 1:1,000 dilution of this antibody detected MICA in one million HeLa cells.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -10°C to -25°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Selective intracellular retention of virally induced NKG2D ligands by the human cytomegalovirus UL16 glycoprotein Stefan A Welte 1 , Christian Sinzger, Stefan Z Lutz, Harpreet Singh-Jasuja, Kerstin Laib Sampaio, Ute Eknigk, Hans-Georg Rammensee, Alexander Steinle Eur J Immunol
33(1)
194-203
2003
Human cytomegalovirus (HCMV) has evolved a multitude of molecular mechanisms to evade the antiviral immune defense of the host. Recently, using soluble recombinant molecules, the HCMV UL16 glycoprotein was shown to interact with some ligands of the activating immunoreceptor NKG2D and, therefore, may also function as a viral immunomodulator. However, the role of UL16 during the course of HCMV infection remained unclear. Here, we demonstrate that HCMV infection of fibroblasts induces expression of all known NKG2D ligands (NKG2DL). However, solely MICA and ULBP3 reach the cellular surface to engage NKG2D, whereas MICB, ULBP1 and ULBP2 are selectively retained in the endoplasmic reticulum by UL16. UL16-mediated reduction of NKG2DL cell surface density diminished NK cytotoxicity. Thus, UL16 functions by capturing activating ligands for cytotoxic lymphocytes that are synthesized in response to HCMV infection.
Functional expression and release of ligands for the activating immunoreceptor NKG2D in leukemia Helmut Rainer Salih 1 , Holger Antropius, Friederike Gieseke, Stefan Zoltan Lutz, Lothar Kanz, Hans-Georg Rammensee, Alexander Steinle Blood
102(4)
1389-96
2003
NKG2D ligands (NKG2DLs) mark malignant cells for recognition by natural killer (NK) cells and cytotoxic T lymphocytes via the activating immunoreceptor NKG2D. This led to the hypothesis that NKG2DLs play a critical role in tumor immune surveillance. The human NKG2DLs MICA and MICB are expressed on tumors of epithelial origin in vivo. For the other recently described set of human NKG2DLs, the UL16-binding proteins (ULBPs), expression in vivo is as yet undefined. In this study we investigated expression and function of NKG2DLs in leukemia using a panel of newly generated NKG2DL-specific monoclonal antibodies. We report that leukemia cells from patients variously express MIC and ULBP molecules on the cell surface with MICA most frequently detected. Patient leukemia cells expressing MICA were lysed by NK cells in an NKG2D-dependent fashion. Sera of patients, but not of healthy donors, contained elevated levels of soluble MICA (sMICA). We also detected increased sMICB levels in patient sera using a newly established MICB-specific enzyme-linked immunosorbent assay. Reduction of leukemia MIC surface expression by shedding may impair NKG2D-mediated immune surveillance of leukemias. In addition, determination of sMICA and sMICB levels may be implemented as a prognostic parameter in patients with hematopoietic malignancies.
